{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:26:45Z","timestamp":1740104805292,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T00:00:00Z","timestamp":1616716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["# 20.254\/2011"],"award-info":[{"award-number":["# 20.254\/2011"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To evaluate the expression of genes and proteins involved in the urethral components: vessels, nerves, and extracellular matrix, in female rats after trauma by vaginal distension (VD) and after electrical stimulation therapy (electrotherapy).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analyzed the urethras of three groups of 18 female rats 30 days posttrauma by VD: control (no interventions); trauma (animals that had VD); and electrotherapy group (those that had VD and were treated with electrical stimulation). We compared the expression of vascular endothelial growth factor (VEGF), nerve growth factor (NGF), collagen types I and III (COL1a1 and COL3a1), and lysyl\u2010oxidase like 1 (LOXL1) among the groups. Real\u2010time reverse transcription\u2010polymerase chain reaction, Western blot, and immunohistochemistry were used for molecule quantification. We used the Kruskal\u2013Wallis test and analysis of variance for statistical analyses with <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 for significance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The COL1a1 gene expression was higher in the electrotherapy group than the trauma group (<jats:italic>p<\/jats:italic>\u2009=\u20090.036). COL3a1, VEGF, NGF, LOXL1 messenger RNA (mRNA) expression did not differ among the groups (<jats:italic>p<\/jats:italic>\u2009\u2265\u20090.05). COL1a1, COL3a1, VEGF, NGF, LOXL1 protein levels did not significantly differ among the groups (<jats:italic>p<\/jats:italic>\u2009\u2265\u20090.05) in Western blot analysis or immunohistochemistry assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Electrotherapy caused a long\u2010term increase in the COL1a1 mRNA level but did not change COL1a1 protein expression or VEGF, NGF, COL3a1, and LOXL1 genes and proteins in the urethras of rats after trauma by VD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24664","type":"journal-article","created":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T14:21:26Z","timestamp":1616768486000},"page":"968-977","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Electrotherapy for urethral modulation: Are extracellular matrix molecules and growth factors potential targets?"],"prefix":"10.1002","volume":"40","author":[{"given":"Suellen M.","family":"Feitosa","sequence":"first","affiliation":[{"name":"Department of Gynecology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2824-5320","authenticated-orcid":false,"given":"Maria A. T.","family":"Bortolini","sequence":"additional","affiliation":[{"name":"Department of Gynecology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisela R. F.","family":"Salerno","sequence":"additional","affiliation":[{"name":"Department of Gynecology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1014-1555","authenticated-orcid":false,"given":"Andreisa P. M.","family":"Bilhar","sequence":"additional","affiliation":[{"name":"Department of Gynecology Universidade Federal de S\u00e3o Paulo  S\u00e3o Paulo Brazil"},{"name":"Department of Gynecology Universidade Federal do Cear\u00e1  Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia S. 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