{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:26:22Z","timestamp":1740104782313,"version":"3.37.3"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["1R01DK124599\u201001","R21DK106554\u201001"],"award-info":[{"award-number":["1R01DK124599\u201001","R21DK106554\u201001"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Gene expression profiling of bladder biopsies in patients with interstitial cystitis\/bladder pain syndrome (IC\/BPS), typically obtained following therapeutic bladder hydrodistention (HOD), is used to improve our understanding of molecular phenotypes. The objective of this study was to determine if the HOD procedure itself impacts the biopsy gene expression profile and, by extension, whether biopsies from non\u2010HOD bladders are appropriate controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Bladder biopsies were obtained just before HOD and immediately following HOD from 10 consecutively recruited IC\/BPS patients undergoing therapeutic HOD. Biopsies were also obtained from four non\u2010IC\/BPS patients who did not undergo HOD (controls). Total RNA was isolated from each of the 24 samples and used to query whole\u2010genome microarrays. Differential gene expression analysis was performed to compare expression profiles of IC\/BPS biopsies before and after HOD, and between IC\/BPS and control biopsies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Principal component analysis\u00a0revealed complete separation between gene expression profiles from IC\/BPS and control samples (<jats:italic>q<\/jats:italic>\u2009\u2264\u20090.05) and while IC\/BPS samples before and after HOD showed no significant differences in expressed genes, 68 transcripts were found to be significantly different between IC\/BPS and control samples (<jats:italic>q<\/jats:italic>\u2009\u2264\u20090.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The bladder HOD procedure itself does not significantly change gene expression within the IC\/BPS patient bladder, a finding that provides evidence to support the use of biopsies from non\u2010IC\/BPS patients that have not undergone HOD as <jats:italic>controls<\/jats:italic> for gene expression studies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24680","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T07:26:20Z","timestamp":1620113180000},"page":"1126-1132","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Hydrodistention does not alter bladder gene expression profiles in patients with non\u2010Hunner lesion interstitial cystitis\/bladder pain syndrome"],"prefix":"10.1002","volume":"40","author":[{"given":"Raymond","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Urology Wake Forest Baptist Health  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey","family":"Schachar","sequence":"additional","affiliation":[{"name":"Florida Robotic and Minimally Invasive Urogynecology  Plantation Florida 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USA"},{"name":"Wake Forest Baptist Health Institute for Regenerative Medicine  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,5,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/865463"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044687"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-015-1021-1"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2011.05.050"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2009.08467.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.03.064"},{"issue":"6","key":"e_1_2_10_8_1","first-page":"624","article-title":"Role of cystoscopy and hydrodistention in the diagnosis of interstitial cystitis\/bladder pain syndrome","volume":"4","author":"Ens 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