{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T01:53:48Z","timestamp":1789610028105,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T00:00:00Z","timestamp":1633564800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>The therapeutic effect of estrogen on interstitial cystitis\/bladder pain syndrome is unclear. We aim to explore the effect of estrogen on bladder overactivity in rats with cyclophosphamide\u2010induced cystitis and its underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In vivo cystometry was used to determine the effect of estrogen on bladder excitability. The effect of estrogen on the expression of P2X3 receptors in bladder epithelium was detected by real\u2010time polymerase chain reaction and western blot. Effect of P2X3 receptors in bladder urothelium on stretch\u2010released adenosine triphosphate was performed by a Flexcell FX5000 Compression system and an Enzyme\u2010Linked Immunosorbent Assay Kit.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Estrogen deprivation significantly increased the urinary frequency, while supplementation with diarylpropionitrile (DPN), an estrogen receptor \u03b2 (ER\u03b2) agonist, alleviated the urinary frequency. 17\u03b2\u2010Estradiol and DPN decreased the expression of P2X3 receptors in urothelium cells which was partially inhibited by ER\u03b2 antagonist 4\u2010[2\u2010phenyl\u20105,7\u2010bis(trifluoromethyl)pyrazolo[1,5\u2010a]pyrimidin\u20103\u2010yl]phenol. Meanwhile, inhibiting the expression of P2X3 receptors by ER\u03b2 agonist or antagonizing the function of P2X3 receptors by selective P2X3 receptor antagonist AF\u2010353 or A\u2010317491 significantly reduced the stretch\u2010released ATP from urothelium cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Estrogen has a direct effect on the regulation of bladder overactivity in rats with cyclophosphamide\u2010induced cystitis by downregulating the expression of bladder epithelial P2X3 receptors through ER\u03b2 and reducing the adenosine triphosphate released from urothelium during bladder filling, thereby inhibiting the generation of the micturition reflex.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24811","type":"journal-article","created":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T00:49:17Z","timestamp":1633826957000},"page":"174-187","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Estrogen inhibits bladder overactivity in rats with cyclophosphamide\u2010induced cystitis via downregulating the expression of P2X3 receptors in bladder epithelium cells"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9569-2625","authenticated-orcid":false,"given":"Xin","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Urology, Second Affiliated Hospital Army Medical University Chongqing Shapingba P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology, Second Affiliated Hospital Army Medical University Chongqing Shapingba P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Urology, Second Affiliated Hospital Army Medical University Chongqing Shapingba P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengshuai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology, Second Affiliated Hospital Army Medical University Chongqing Shapingba P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingzhen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Urology, Second Affiliated Hospital Army Medical University Chongqing Shapingba P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingyou","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Urology, Second Affiliated Hospital Army Medical University Chongqing Shapingba P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6700-7096","authenticated-orcid":false,"given":"Jie","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Urology, Second Affiliated Hospital Army Medical University Chongqing Shapingba P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23072"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2004.06.012"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2015.01.086"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2012.217"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.22464"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2008.10.104"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(00)00441-X"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2015.1063119"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000176747.40242.3d"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/iju.12308"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(01)01639-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOU.0b013e3283476ea2"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.10.106"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/35039519"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-15-05670.2001"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.13003"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0b013e328353fabc"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11302-010-9212-9"},{"key":"e_1_2_9_20_1","first-page":"811","article-title":"Estrogen altered facial mechanical pain threshold and trigeminal P2X3 receptor expression","volume":"32","author":"Yu LH","year":"2011","journal-title":"Neuro Endocrinol Lett"},{"key":"e_1_2_9_21_1","first-page":"113","article-title":"Animal models of overactive bladder: cyclophosphamide (CYP)\u2010induced cystitis in rats","volume":"48","author":"Juszczak K","year":"2007","journal-title":"Folia Med Cracov"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28829"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.21180"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3127-9_1"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703410104"},{"key":"e_1_2_9_26_1","article-title":"Interstitial cystitis or painful bladder syndrome in a premenopausal female precipitated by oral combined contraceptives","volume":"12","author":"Thompson A","year":"2020","journal-title":"Cureus"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-52531-9"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2015.0427"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2005.06.099"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.24811","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/nau.24811","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.24811","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T08:27:05Z","timestamp":1692952025000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.24811"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,7]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/nau.24811"],"URL":"https:\/\/doi.org\/10.1002\/nau.24811","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,7]]},"assertion":[{"value":"2021-08-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}