{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T23:33:14Z","timestamp":1770679994693,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Ketamine\u2010induced cystitis (KIC) is a disease caused by ketamine that can cause lower urinary tract symptoms (LUTS). Its end\u2010stage is bladder contracture, which is related to bladder fibrosis and poses a serious burden to patient lives.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We established a KIC model in female Sprague Dawley rats and verified bladder fibrosis in the model by Masson trichrome staining and western blot analysis. The bladders of the rats from the ketamine and control groups were used to perform transcriptome analysis. In particular, association analysis with metabolomics was also used to determine the potential mechanisms of ketamine\u2010induced bladder fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 685 differentially expressed messenger RNAs, 71 differentially expressed long noncoding RNAs, 23 differentially expressed microRNAs, and 68 differentially expressed circular RNAs were identified. We found that ribosome, Wnt signaling, vascular endothelial growth factor signaling, cytoskeleton organization, and cytoskeletal protein binding may be potential pathways in ketamine\u2010induced bladder fibrosis as identified by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. In addition, the mitogen\u2010activated protein kinase pathway appeared to be closely related to the development of ketamine\u2010induced bladder fibrosis according to association analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In this study, using transcriptomic and correlation analyses of metabolomics, we identified pathways that may be potential targets for the prevention and treatment of ketamine\u2010induced bladder fibrosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24892","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T11:30:02Z","timestamp":1645011002000},"page":"765-776","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Transcriptomic analysis of bladder tissue in a rat model of ketamine\u2010induced bladder fibrosis"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0859-1960","authenticated-orcid":false,"given":"Haozhen","family":"Li","sequence":"first","affiliation":[{"name":"Department of Urology, Xiangya Hospital Central South University  Changsha Hunan China"},{"name":"Department of Urology The second hospital of Dalian medical university Dalian Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5244-383X","authenticated-orcid":false,"given":"Kaixuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology, Xiangya Hospital Central South University  Changsha Hunan China"},{"name":"Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4042-3959","authenticated-orcid":false,"given":"Quan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Urology, Xiangya Hospital Central South University  Changsha Hunan China"},{"name":"Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0693-8748","authenticated-orcid":false,"given":"Zhengyan","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Urology, Xiangya Hospital Central South University  Changsha Hunan China"},{"name":"Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1980-2866","authenticated-orcid":false,"given":"Zhao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology, Xiangya Hospital Central South University  Changsha Hunan China"},{"name":"Provincial Laboratory for Diagnosis and Treatment of Genitourinary System Disease, National Clinical Research Center for Geriatric Disorders, Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0897190014525754"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.12363"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-994851"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/iju.12841"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2007.01.038"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00345-016-1759-9"},{"key":"e_1_2_11_8_1","first-page":"10","article-title":"Studying smaller and neglected organisms in modern evolutionary venomics implementing RNASeq (transcriptomics): a critical guide","volume":"10","author":"Reumont B","year":"2018","journal-title":"Toxins"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201800169"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-020-02595-x"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2018.11.002"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652411794859250"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.21037\/tau-20-1202"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.119.039596"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/eci.13153"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00063.2015"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00479.2019"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2015.10.013"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2017.12.001"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-70243-3"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.129177"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2016.06.006"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2017.500"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2018.05.002"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2016.133"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201701118R"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26959"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.21287"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/YCT.0b013e31824d1d02"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2010.06.013"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12017-009-8070-5"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2011.162"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddy231"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmg.2019.103736"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-020-06771-y"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-020-01652-5"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00287.2006"},{"key":"e_1_2_11_38_1","first-page":"38","article-title":"Myofibroblast\u2010mediated contraction","volume":"27","author":"Kattan WM","year":"2017","journal-title":"J Coll Phys Surg\u2010\u2010Pak"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.5482"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.140796"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.126834"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-020-01235-0"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.24892","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/nau.24892","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.24892","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T21:03:36Z","timestamp":1692997416000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.24892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,16]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1002\/nau.24892"],"URL":"https:\/\/doi.org\/10.1002\/nau.24892","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,16]]},"assertion":[{"value":"2021-09-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}