{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T08:14:24Z","timestamp":1781597664073,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Bladder outlet obstruction (BOO) is a common problem that can affect bladder structure and function. Currently, there is no effective drugs available to prevent BOO\u2010induced remodeling. Previous reports have demonstrated that the pathogenesis of BOO is associated with macrophage infiltration and polarization, which is physiologically dependent on colony\u2010stimulating factor 1 receptor (CSF\u20101R) activation. Here\u00a0we utilized a highly selective CSF\u20101R inhibitor, GW2580, to determine its preventive effects on BOO\u2010induced remodeling.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 24 Sprague\u2013Dawley rats were randomly divided into sham, BOO\u2009+\u2009vehicle, and BOO\u2009+\u2009GW2580 group. GW2580 or vehicle control was administrated by oral gavage at daily doses of 40\u2009mg\/kg for 6 weeks. Bladder samples were collected for histopathology, immunohistochemistry, immunofluorescence, western blotting, and flow cytometry analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results demonstrated that bladder fibrosis was ameliorated by GW2580 compared with the vehicle group (22.01%\u2009\u00b1\u20095.13% vs. 32.15%\u2009\u00b1\u20097.24%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Furthermore, treatment with GW2580 induced an inhibition of macrophage infiltration (4.41%\u2009\u00b1\u20091.28% vs. 13.57%\u2009\u00b1\u20093.42%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and M2 macrophage polarization (10.67%\u2009\u00b1\u20094.15% vs. 28.59%\u2009\u00b1\u20096.38%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). There was also a decrease of profibrotic F4\/80<jats:sup>+<\/jats:sup>\u00a0\u03b1\u2010smooth muscle actin<jats:sup>+<\/jats:sup> (\u03b1\u2010SMA<jats:sup>+<\/jats:sup>) macrophage to myofibroblast transition (9.11%\u2009\u00b1\u20092.58% vs. 17.33%\u2009\u00b1\u20094.01%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and CD163<jats:sup>+<\/jats:sup>TGF\u2010\u03b21<jats:sup>+<\/jats:sup> cells (7.68%\u2009\u00b1\u20092.10% vs. 14.17%\u2009\u00b1\u20094.09%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) in the GW2580 group when compared with the vehicle group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In summary, our findings showed that GW2580 is a worthwhile candidate for a follow\u2010up study to test in the treatment of BOO\u2010induced remodeling.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24896","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T11:21:14Z","timestamp":1645010474000},"page":"787-796","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The preventive effects of colony\u2010stimulating factor 1 receptor (CSF\u20101R) inhibition on bladder outlet obstruction induced remodeling"],"prefix":"10.1002","volume":"41","author":[{"given":"Wei","family":"Wang","sequence":"first","affiliation":[{"name":"Laboratory of Reconstructive Urology, Department of Urology, Institute of Urology, West China Hospital Sichuan University Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjin","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of General Practice, West China Hospital Sichuan University Chengdu 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