{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T02:23:01Z","timestamp":1778811781955,"version":"3.51.4"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T00:00:00Z","timestamp":1658534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870521"],"award-info":[{"award-number":["81870521"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82070786"],"award-info":[{"award-number":["82070786"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004921","name":"Shanghai Jiao Tong University","doi-asserted-by":"publisher","award":["TMSZ\u20102020\u2010208"],"award-info":[{"award-number":["TMSZ\u20102020\u2010208"]}],"id":[{"id":"10.13039\/501100004921","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To examine the effects of the selective 5\u2010HT<jats:sub>1A<\/jats:sub> receptor agonist, NLX\u2010112, on urethral function in streptozotocin\u2010induced diabetic rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Female Sprague\u2013Dawley rats (<jats:italic>n<\/jats:italic>\u2009=\u200932) were divided into two groups: rats with type 1 diabetes mellitus (T1DM) and age\u2010matched normal control rats (NC). T1DM was induced by intraperitoneal injection of streptozotocin (65\u2009mg\/kg). Isovolumetric cystometry and urethral perfusion pressure (UPP) were evaluated 10 weeks postinjection in rats (<jats:italic>n<\/jats:italic>\u2009=\u20099 per group). The selective 5\u2010HT<jats:sub>1A<\/jats:sub> receptor antagonist, WAY\u2010100635 maleate salt, was administered after NLX\u2010112 hydrochloride dose\u2013response curve was generated (intravenously). The remaining rats were used for immunofluorescence and Western blot assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to controls, type 1 diabetic rats (T1D rats) had lower maximal intravesical pressure (IP max) and UPP changes. In T1D rats, NLX\u2010112 hydrochloride (0.003\u20131.0\u2009mg\/kg) induced dose\u2010dependent decreases in UPP nadir, IP max, high\u2010frequency oscillations (HFOs) rate; and increases in UPP change and HFOs amplitude. WAY\u2010100635 maleate salt (0.3\u2009mg\/kg) partially or completely reversed the NLX\u2010112\u2010induced changes. Immunofluorescence revealed that 5\u2010HT<jats:sub>1A<\/jats:sub> receptors were found in the L6\u2010S1 spinal cord dorsolateral nucleus, but the expression was significantly higher in the T1D rats. Additionally, Western blot showed there were significantly more 5\u2010HT<jats:sub>1A<\/jats:sub> receptors in the ventral L6\u2010S1 spinal cord of T1D rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Urethral dysfunction in T1D rats was improved by NLX\u2010112. 5\u2010HT<jats:sub>1A<\/jats:sub> receptors were upregulated in the dorsolateral nucleus of L6\u2010S1 spinal cord in T1D rats. These findings suggest that NLX\u2010112 may constitute a novel therapeutic strategy to treat diabetic urethral dysfunction.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.24993","type":"journal-article","created":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T19:00:30Z","timestamp":1658602830000},"page":"1528-1538","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Improvement of urethral dysfunction by 5\u2010HT<sub>1A<\/sub>\u00a0receptor agonist NLX\u2010112 in diabetic rats"],"prefix":"10.1002","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0109-1501","authenticated-orcid":false,"given":"Mingzhuo","family":"Li","sequence":"first","affiliation":[{"name":"Department of Urology Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai 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