{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T16:17:21Z","timestamp":1772209041106,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T00:00:00Z","timestamp":1665187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To explore an efficient preventive strategy for radiation cystitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We instilled IR\u2010780 into the bladders of rats 1\u2009h before bladder irradiation, and its bio\u2010distribution was observed at different times. Bladders were then examined for pathogenic alterations and inflammation levels by day 3 and week 12 postirradiation, and the functional characteristics of the bladder were tested via cystometry by week 12. Human uroepithelial sv\u2010huc\u20101 cells were used to determine the effect of IR\u2010780 on cell viability, regardless of irradiation. We measured the intracellular levels of oxidative stress, DNA damage, apoptosis proportion, and the expression of antioxidant proteases and apoptotic caspases in IR\u2010780 pretreated cells after radiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IR\u2010780 is localized in the urothelium after intravesical instillation in vivo. Ionizing radiation could induce acute impairment of the bladder urothelium and inflammation in the bladder on day 3. Fibrosis of the irradiated bladder progressed and eventually affected voiding function at 12 weeks. Treatment with IR\u2010780 before irradiation ameliorated these changes. In vitro, IR\u2010780 protected against cell viability and apoptosis of sv\u2010huc\u20101 cells after irradiation. Additionally, IR\u2010780 may assist in eliminating reactive oxygen species and repairing irradiation\u2010induced DNA damage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data indicate that IR\u2010780 can be used before irradiation to prevent acute urinary mucosal injury and late bladder dysfunction. Moreover, early urothelial impairment plays a significant role in radiation cystitis development.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.25056","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T09:34:52Z","timestamp":1665221692000},"page":"40-48","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Intravesical IR\u2010780 instillation prevents radiation cystitis by protecting urothelial integrity"],"prefix":"10.1002","volume":"42","author":[{"given":"Jinjin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chongxing","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heping","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianwu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaofeng","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linyong","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuandi","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Zhi","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunmeng","family":"Shi","sequence":"additional","affiliation":[{"name":"Institute of Rocket Force Medicine, State Key Laboratory of Trauma, Burns and Combined Injury Army Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6431-8251","authenticated-orcid":false,"given":"Weibing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology The Third Affiliated Hospital of Chongqing Medical University Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,10,8]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21654"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15149"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.294.10.1233"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2010.23"},{"key":"e_1_2_11_6_1","first-page":"CD001773","article-title":"Non\u2010surgical interventions for late radiation cystitis in patients who have received radical radiotherapy to the pelvis","volume":"3","author":"Denton AS","year":"2002","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)00977-8"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12894-015-0046-1"},{"issue":"7","key":"e_1_2_11_9_1","first-page":"328","article-title":"Intravesical glycosaminoglycan replenishment with chondroitin sulphate in chronic forms of cystitis. 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