{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T09:53:26Z","timestamp":1773741206121,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T00:00:00Z","timestamp":1698624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the effect of an injection of botulinum toxin A (BoNT\/A) at the epicenter of the spinal cord injury (SCI) site on the recovery of lower urinary tract function in female rats with thoracic SCI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Twenty\u2010four female Wistar rats with Sham (laminectomy at T8\/T9 level) or SCI (at T8\/T9; 30\u2009g compression for 5\u2009s) were assigned into Sham\u2010SS (injected with 5\u2009\u00b5L of saline solution), Sham\u2010BoNT\/A (injected with 15\u2009pg\/rat, equivalent to 7.5\u2009Units\/kg of BoNT\/A in 5\u2009\u00b5L volume), SCI\u2010SS (injured and injected with saline), SCI\u2010BoNT\/A (injured and injected with BoNT\/A), <jats:italic>N<\/jats:italic>\u2009=\u20096 per group. Weekly evaluation of stereotyped micturition behavior, hind\u2010limb nociception, and locomotor activity was performed 1 week before and during 6 weeks after surgery. Subsequently, all groups underwent simultaneous electromyography of the external urethral sphincter (EUS\u2010EMG) and cystometric (CMG) studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A compression SCI at the T8\/T9 thoracic level significantly impairs sensory and locomotive functions, as well as stereotyped micturition behavior. However, these impairments were improved by BoNT\/A injection after SCI. Neither injections of saline solution nor BoNT\/A had an appreciable effect on the same parameters evaluated in the Sham groups. The combined EUS\u2010EMG and CMG evaluations revealed important improvements of lower urinary tract physiology, particularly a reduction in the frequency of non\u2010voiding contractions and the properties of EUS bursting activity indicated as the amplitude of the EUS\u2010EMG signal and duration of burst electrical activity during effective voiding.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The severe impairments on sensory and locomotive functions as well stereotyped micturition caused by an SCI could be potentially attenuated by an injection of a small amount of BoNT\/A directly into the epicenter of the SCI region. A reduction in the release of neurotoxic neurotransmitters requiring the SNARE complex may be the mechanism triggered by BoNT\/A to reduce neurotoxicity and hyperexcitability created in the SCI area to improve the survival of spinal cord cells involved in micturition.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.25311","type":"journal-article","created":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T09:15:45Z","timestamp":1698657345000},"page":"246-257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Improvement of neurogenic urinary dysfunctions in female rats treated with an injection of botulinum toxin A at the epicenter of the spinal cord injured site"],"prefix":"10.1002","volume":"43","author":[{"given":"Cynthia K.","family":"Gandara","sequence":"first","affiliation":[{"name":"Centro Universitario del Norte Universidad de Guadalajara Colotl\u00e1n Jalisco M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose L.","family":"Palacios","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias para el Desarrollo Humano Universidad Aut\u00f3noma de Tlaxcala Tlaxcala Tlaxcala Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1257-7723","authenticated-orcid":false,"given":"J.","family":"Luis Quintanar","sequence":"additional","affiliation":[{"name":"Centro de Ciencias B\u00e1sicas Universidad Aut\u00f3noma de Aguascalientes Aguascalientes Aguascalientes M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1927-4103","authenticated-orcid":false,"given":"Yingchun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering University of Houston Houston Texas 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