{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T00:05:47Z","timestamp":1755216347185,"version":"3.43.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T00:00:00Z","timestamp":1753315200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>To characterize the urinary microbiome in women performing clean intermittent self\u2010catheterization (CISC) and explore microbial changes associated with transitioning from single\u2010use to reusable catheters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This microbiome study of the COMPaRE trial included female CISC patients with urinary retention randomized to either single\u2010use or reusable catheters. Self\u2010catheterized urine samples were collected at baseline and Week 6 for standard culture and 16S rRNA microbiome analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 28 patients (12 reusable, 16 single\u2010use) were included, with a median age of 48 years (IQR 32\u221260); 67.9% had neurogenic lower urinary tract dysfunction. <jats:italic>Escherichia\u2010Shigella<\/jats:italic> (36.3%) and <jats:italic>Lactobacillus<\/jats:italic> (22.8%) were the most prevalent genera. PERMANOVA identified significant effects of age (<jats:italic>p<\/jats:italic>\u2009=\u20090.003), menopausal status (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), and catheterization cause (<jats:italic>p<\/jats:italic>\u2009=\u20090.003) on microbiome composition. Transitioning to reusable catheters was associated with significant reductions in <jats:italic>Escherichia\u2010Shigella<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and <jats:italic>Aerococcus<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), and increases in <jats:italic>Veillonella<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and <jats:italic>Finegoldia<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). No significant changes were observed in urine culture results (<jats:italic>p<\/jats:italic>\u2009=\u20090.250), alpha diversity as measured by the Shannon index (<jats:italic>p<\/jats:italic>\u2009=\u20090.862), or beta diversity as assessed by Bray\u2010Curtis distance (<jats:italic>p<\/jats:italic>\u2009=\u20090.096).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>Escherichia\u2010Shigella<\/jats:italic> and <jats:italic>Lactobacillus<\/jats:italic> were the most abundant genera in the urinary microbiome of women performing CISC. Age, menopausal status, and catheterization cause significantly influenced microbiome composition. Although specific microbial shifts were observed following transition to reusable catheters, no significant changes in overall microbial diversity were detected. Larger, well\u2010powered studies are needed to validate these results and assess their potential clinical implications. <jats:bold>Trial Registration:<\/jats:bold> Clinical Trial RegistrationNL\u2010OMON54700\/NL8296.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nau.70119","type":"journal-article","created":{"date-parts":[[2025,7,25]],"date-time":"2025-07-25T05:40:11Z","timestamp":1753422011000},"page":"1474-1483","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Urinary Microbiome in Women Using Single\u2010Use Versus Reusable Catheters for Intermittent Catheterization: An Exploratory Substudy of the COMPaRE Trial"],"prefix":"10.1002","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0947-052X","authenticated-orcid":false,"given":"Felice Emanuela Esp\u00e8rance","family":"van Veen","sequence":"first","affiliation":[{"name":"Department of Urology Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaleh Esmi","family":"Serkani","sequence":"additional","affiliation":[{"name":"Department of Urology Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5620-3275","authenticated-orcid":false,"given":"Sophie","family":"Berendsen","sequence":"additional","affiliation":[{"name":"Department of Urology Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0416-999X","authenticated-orcid":false,"given":"Robert","family":"Kraaij","sequence":"additional","affiliation":[{"name":"Laboratory of Population Genomics, Department of Internal Medicine Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lonneke","family":"Bode","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Infectious Diseases Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John Philip","family":"Hays","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology &amp; Infectious Diseases Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeroen Ronald","family":"Scheepe","sequence":"additional","affiliation":[{"name":"Department of Urology Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9354-7395","authenticated-orcid":false,"given":"Bertil Freddo Maarten","family":"Blok","sequence":"additional","affiliation":[{"name":"Department of Urology Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,24]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2024.03.026"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2017.01.075"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.23562"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2023.11.004"},{"issue":"10","key":"e_1_2_11_6_1","article-title":"Intermittent Catheter Techniques, Strategies and Designs for Managing Long\u2010Term Bladder Conditions","volume":"10","author":"Prieto J. 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