{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T04:09:21Z","timestamp":1778818161608,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neurourology and Urodynamics"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Although TRPV1 is present in detrusor smooth muscle (DSM), interstitial cells of Cajal, and urothelium, its physiological role remains unclear. The present study demonstrated the presence of TRPV1 mRNA in goat DSM, including the physiological effects of the TRPV1 channel and its modulation by nitric oxide.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Species identity was confirmed by amplification of the mitochondrial 16S rRNA gene from the genomic DNA of the goat (\n                      <jats:italic>Capra hircus<\/jats:italic>\n                      ) DSM, and the samples were subjected to RT\u2010PCR for TRPV1 gene expression. For functional studies, DSM strips (2\u20133 \u00d7 4\u20135\u2009mm) from freshly slaughtered male goats (6\u20138 months) were mounted in organ baths. Capsaicin (1 nM\u201310\u2009\u00b5M) was applied cumulatively to DSM precontracted with carbachol (CCh, 10\u2009\u00b5M) or high K\u207a solution (30\u2009mM), in the absence or presence of BCTC (1\u2009\u00b5M), yohimbine (1\u2009\u00b5M), glibenclamide (10\u2009\u00b5M), sodium nitroprusside (SNP, 10\u2009\u00b5M),\n                      <jats:sc>l<\/jats:sc>\n                      \u2010NAME (10\u2009\u00b5M), or ODQ (1\u2009\u00b5M).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      RT\u2010PCR confirmed TRPV1 mRNA expression. Capsaicin produced maximal relaxation (Rmax) of 45.5% (95% CI\u2009=\u200941.9\u201349.1) and 42.8 (95% CI\u2009=\u200937.1\u201348.5) in CCh\u2010 and high K\u207a\u2010 contracted DSM, respectively. R\n                      <jats:sub>max<\/jats:sub>\n                      was reduced to 0% and 3.9% (95% CI\u2009=\u20091.4\u20136.4) by BCTC; 6.8% (95% CI\u2009=\u20092.7\u201310.9) and 27.3% (95% CI\u2009=\u200910.1\u201344.5) by yohimbine, 4.7% (95% CI\u2009=\u20091.8\u20137.6) and 28.5% (95% CI\u2009=\u20099.7\u201347.3) by glibenclamide in CCh\u2010 and K\u207a30\u2010PSS \u2010 contracted DSM, respectively. SNP enhanced the capsaicin\u2010evoked\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sub>max<\/jats:sub>\n                      to 76.3 (95% CI\u2009=\u200955\u201397.6) and 85.2% (95% CI\u2009=\u200950.2\u2013120.2), whereas\n                      <jats:sc>l<\/jats:sc>\n                      \u2010NAME decreased it to 26.7% (95% CI\u2009=\u200911.1\u201342.3) and 11.8% (95% CI 4.8\u201318.8), and ODQ to 12.4% (95% CI\u2009=\u20094.8\u201320.0) and 7.7 (95% CI\u2009=\u20093.2\u201312.2) in CCh\u2010contracted and high K\u207a\u2010depolarized DSM, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The present study demonstrates that goat DSM expresses functional TRPV1 channels and capsaicin elicits relaxation by activating these channels. The NO donor SNP further potentiates the capsaicin\u2010induced relaxation through a NO\u2010cGMP signalling pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/nau.70263","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T10:30:37Z","timestamp":1773225037000},"page":"835-844","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sodium Nitroprusside Potentiates Capsaicin\u2010Activated TRPV1\u2010mediated Relaxation of Goat Detrusor Smooth Muscle"],"prefix":"10.1002","volume":"45","author":[{"given":"Khumtya","family":"Debbarma","sequence":"first","affiliation":[{"name":"Department of Veterinary Pharmacology &amp; Toxicology College of Veterinary Science, AVFU, Khanapara Guwahati India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luitmoni","family":"Barkalita","sequence":"additional","affiliation":[{"name":"Department of Animal Biotechnology College of Veterinary Science, AVFU, Khanapara Guwahati India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dilip. K.","family":"Deka","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pharmacology &amp; Toxicology College of Veterinary Science, AVFU, Khanapara Guwahati India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,11]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"383","article-title":"The Dual, Sensory, and Efferent Function of the Capsaicin\u2010Sensitive Primary Sensory Nerves in the Bladder and Urethra. The Autonomic Nervous System","volume":"3","author":"Maggi C. A.","year":"1993","journal-title":"Nervous Control of the Urogenital System"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2040-4603.2015.tb00596.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/39807"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-84734-4"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/medsci7050067"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09650.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1159\/000436976"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.275.2.F226"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01191.2007"},{"key":"e_1_2_9_11_1","first-page":"185","article-title":"Effect of KATP Channel Openers on Myogenic and Neurogenic Responses in Goat Urinary Bladder","volume":"45","author":"Vijayakumar C.","year":"2007","journal-title":"Indian Journal of Experimental Biology"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aju.2011.12.009"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0705794"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1992.sp019157"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)66115-9"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2125.1993.tb04152.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.20517"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410X.2002.02795.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2013.262410"},{"issue":"2","key":"e_1_2_9_20_1","first-page":"110","article-title":"Nitric Oxide Produced Inhibitory Effect Against Carbachol\u2010Induced Contraction in the Detrusor Muscles of Goat","volume":"80","author":"Baruah R. K.","year":"2010","journal-title":"Indian Journal of Animal Sciences"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/nau.21247"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2023.1249560"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00064.2017"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2022.08.025"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27031031"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2006.06.020"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP273455"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200310000-00009"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0259OC"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1745-7262.2007.00291.x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00232-008-9121-9"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00515.2005"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.107.043323"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.10.021"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-827X(01)01048-5"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2003.11.039"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0191032"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1998.275.2.F226"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00210-002-0630-2"}],"container-title":["Neurourology and Urodynamics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.70263","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/nau.70263","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nau.70263","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T03:22:23Z","timestamp":1778815343000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/nau.70263"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,11]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["10.1002\/nau.70263"],"URL":"https:\/\/doi.org\/10.1002\/nau.70263","archive":["Portico"],"relation":{},"ISSN":["0733-2467","1520-6777"],"issn-type":[{"value":"0733-2467","type":"print"},{"value":"1520-6777","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,11]]},"assertion":[{"value":"2025-09-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}