{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T18:18:57Z","timestamp":1790965137949,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,7,10]],"date-time":"2009-07-10T00:00:00Z","timestamp":1247184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An unassigned and prominent resonance in the region from <jats:italic>\u03b4<\/jats:italic> 2.0\u20132.1\u2009ppm has frequently been found in the <jats:italic>in vivo<\/jats:italic> MR spectra of cancer patients. We demonstrated the presence of this resonance with <jats:italic>in vivo<\/jats:italic> MRS in the cyst fluid of a patient with an ovarian tumor. <jats:sup>1<\/jats:sup>H\u2010NMRS on the aspirated cyst fluid of this patient confirmed the observation. A complex of resonances was observed between 2.0 and 2.1\u2009ppm. It was also present in 11 additional ovarian cyst fluid samples randomly chosen from our biobank. The resonance complex was significantly more prominent in samples from mucinous tumors than in samples from other histological subtypes. A macromolecule (&gt;10\u2009kDa) was found responsible for this complex of resonances. A correlation spectroscopy (COSY) experiment revealed cross peaks of two different types of bound sialic acid suggesting that <jats:italic>N<\/jats:italic>\u2010glycans from glycoproteins and\/or glycolipids cause this resonance complex. In the literature, plasma <jats:italic>\u03b1<\/jats:italic>\u20101 acid glycoprotein (AGP), known for its high content of <jats:italic>N<\/jats:italic>\u2010linked glycans, has been suggested to contribute to the <jats:italic>\u03b4<\/jats:italic> 2.0\u20132.1 spectral region. The AGP cyst fluid concentration did not correlate significantly with the peak height of the <jats:italic>\u03b4<\/jats:italic> 2.0\u20132.1 resonance complex in our study. AGP may be partly responsible for the resonance complex but other <jats:italic>N<\/jats:italic>\u2010acetylated glycoproteins and\/or glycolipids also contribute. After deproteinization of the cyst fluid, <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:sc>L<\/jats:sc>\u2010aspartic acid (NAA) was found to contribute significantly to the signal in this spectral region in three of the 12 samples. GC\u2010MS independently confirmed the presence of NAA in high concentration in the three samples, which all derived from benign serous tumors. We conclude that both NAA and <jats:italic>N<\/jats:italic>\u2010acetyl groups from glycoproteins and\/or glycolipids may contribute to the <jats:italic>\u03b4<\/jats:italic> 2.0\u20132.1\u2009ppm resonance complex in ovarian cyst fluid. This spectral region seems to contain resonances from biomarkers that provide relevant clinical information on the type of ovarian tumor. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/nbm.1417","type":"journal-article","created":{"date-parts":[[2009,7,10]],"date-time":"2009-07-10T19:29:02Z","timestamp":1247254142000},"page":"1093-1099","source":"Crossref","is-referenced-by-count":36,"title":["<i>N<\/i>\u2010acetyl resonances in <i>in vivo<\/i> and <i>in vitro<\/i> NMR spectroscopy of cystic ovarian tumors"],"prefix":"10.1002","volume":"22","author":[{"given":"Eva","family":"Kolwijck","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Udo F.","family":"Engelke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marinette","family":"van der Graaf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arend","family":"Heerschap","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henk 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