{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T03:48:24Z","timestamp":1777952904908,"version":"3.51.4"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,9,9]],"date-time":"2009-09-09T00:00:00Z","timestamp":1252454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A genetic deficiency of lysosomal alpha\u2010mannosidase causes the lysosomal storage disease alpha\u2010mannosidosis (AMD), in which oligosaccharide accumulation occurs in neurons and glia. The purpose of this study was to evaluate the role of magnetic resonance spectroscopy (MRS) in detecting the oligosaccharide accumulation in AMD. Five cats with AMD and eight age\u2010matched normal cats underwent <jats:italic>in vivo<\/jats:italic> MRS studies with a single voxel short echo time (20\u2009ms) STEAM spectroscopy sequence on a 4.7T magnet. Two voxels were studied in each cat, from the cerebellar vermis and the occipital cortex. Metabolites of brain samples from these regions were extracted with perchloric acid and analyzed by high resolution NMR spectroscopy. A significantly elevated unresolved resonance signal between 3.4 and 4. ppm was observed in the cerebellar vermis and occipital cortex of all AMD cats, which was absent in normal cats. This resonance was shown to be from carbohydrate moieties by high resolution NMR of tissue extracts. Resonances from the Glc\u2010NAc group (1.8\u20132.2\u2009ppm) along with anomeric proton signals (4.6\u20135.4\u2009ppm) from undigested oligosaccharides were also observed in the extract spectra from AMD cats. This MRS spectral pattern may be a useful biomarker for AMD diagnosis as well as for assessing responses to therapy. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/nbm.1430","type":"journal-article","created":{"date-parts":[[2009,9,9]],"date-time":"2009-09-09T10:09:58Z","timestamp":1252490998000},"page":"74-79","source":"Crossref","is-referenced-by-count":26,"title":["Magnetic resonance spectroscopy of the occipital cortex and the cerebellar vermis distinguishes individual cats affected with alpha\u2010mannosidosis from normal cats"],"prefix":"10.1002","volume":"23","author":[{"given":"Sergey","family":"Magnitsky","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles H.","family":"Vite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward J.","family":"Delikatny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Pickup","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne","family":"Wehrli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H.","family":"Wolfe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harish","family":"Poptani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,9,9]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"43","volume-title":"Neurogenetics: Scientific and Clinical Advances","author":"Pierson TM","year":"2005"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2001.1"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20392"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.3174\/ajnr.A0791"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1740-8261.2001.tb00896.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.2522\/ptj.20060149"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22228"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rcl.2004.07.004"},{"key":"e_1_2_1_10_2","first-page":"1958","article-title":"Proton MR spectroscopy of mitochondrial diseases: analysis of brain metabolic abnormalities and their possible diagnostic relevance","volume":"24","author":"Bianchi MC","year":"2003","journal-title":"Am J Neuroradiol."},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-75587-8_1"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200103000-00008"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000269782.80107.fe"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00731639"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(91)80027-Z"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3280863"},{"key":"e_1_2_1_17_2","first-page":"346","volume-title":"Methods of Enzymatic Analysis","author":"Trautschold I","year":"1983"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.16.9164"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.16.9154"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2330899"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000143057.25471.4F"},{"key":"e_1_2_1_22_2","first-page":"3507","volume-title":"Metabolic Basis of Inherited Disease","author":"Thomas GH","year":"2001"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2005.227.570"}],"container-title":["NMR in Biomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnbm.1430","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.1430","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T11:25:56Z","timestamp":1760959556000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/nbm.1430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,9]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1002\/nbm.1430"],"URL":"https:\/\/doi.org\/10.1002\/nbm.1430","archive":["Portico"],"relation":{},"ISSN":["0952-3480","1099-1492"],"issn-type":[{"value":"0952-3480","type":"print"},{"value":"1099-1492","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,9]]}}}