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We evaluated the compatibility of these two techniques by obtaining <jats:sup>31<\/jats:sup>P NMR spectra of iron\u2010labeled and unlabeled immobilized C2C12 myoblast cells in vitro. Broadened but usable <jats:sup>31<\/jats:sup>P spectra were obtained and peak area ratios of resonances corresponding to intracellular metabolites showed no significant differences between labeled and unlabeled cell populations. We conclude that <jats:sup>31<\/jats:sup>P NMR spectra can be obtained from cells labeled with sufficient iron to permit visualization by <jats:sup>1<\/jats:sup>H imaging protocols and that these spectra have sufficient quality to be used to assess metabolic status. This result introduces the possibility of using localized <jats:sup>31<\/jats:sup>P MRS to evaluate the viability of iron\u2010labeled therapeutic cells as well as surrounding host tissue in vivo. 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