{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:29:57Z","timestamp":1761006597092,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":5946,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[1989,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mice with subcutaneous RIF\u20101 tumors were anesthetized with sodium pentobarbital (PB) or ketamine plus acepromazine (KA) before acquisition of <jats:italic>in vivo<\/jats:italic> <jats:sup>31<\/jats:sup>P nuclear magnetic resonance (NMR) spectra from tumors. The area of the inorganic phosphate resonance was significantly greater (relative to phosphomonoesters or the \u03b1\u2010phosphate resonance of nucleoside triphosphate) in spectra obtained under PB anesthesia, suggesting that the hypoxic fraction of the tumor increased following PB anesthesia. <jats:italic>In vivo<\/jats:italic> near\u2010infrared laser spectroscopy directly demonstrated that tumor oxyhemoglobin was reduced by more than 20% following PB but was not significantly affected by KA. Total hemoglobin (tumor blood volume) was reduced by 11% following PB anesthesia, but was not significantly affected by KA. Tumor growth delay induced by \u03b3\u2010irradiation was shorter when tumors were irradiated under PB anesthesia than when irradiated under KA, showing that PB anesthesia had a radioprotective effect. These studies demonstrate that both the <jats:sup>31<\/jats:sup>P NMR and near infrared methods can detect metabolic or physiological changes associated with an increase in tumor radioresistance (i.e., an increase in the radiobiological hypoxic fraction).<\/jats:p>","DOI":"10.1002\/nbm.1940020302","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T07:35:34Z","timestamp":1172993734000},"page":"87-92","source":"Crossref","is-referenced-by-count":23,"title":["<sup>31<\/sup>P NMR spectroscopic and near infrared spectrophotometric studies of effects of anesthetics on <i>In vivo<\/i> RIF\u20101 tumors. relationship to tumor radiosensitivity"],"prefix":"10.1002","volume":"2","author":[{"given":"R. Grant","family":"Steen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy","family":"Bowser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janna P.","family":"Wehrle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jerry D.","family":"Glickson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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