{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:05:40Z","timestamp":1791079540965,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,10,21]],"date-time":"2005-10-21T00:00:00Z","timestamp":1129852800000},"content-version":"vor","delay-in-days":5682,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[1990,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    202 MHz\n                    <jats:sup>31<\/jats:sup>\n                    P NMR (11.7 T) was used to study the effects of culture medium pH on the levels of phosphate metabolites in three human tumor cell lines (XP29MAmal, a malignant xeroderma pigmentosum; CX\u20101, a colon carcinoma; KB, a squamous cell carcinoma of the oral cavity). Cells were cultured in Roux flasks in HAM's F\u201012 medium, and the pH was varied with the final medium change. After harvesting, 1\u20135 \u00d7 10\n                    <jats:sup>8<\/jats:sup>\n                    cells were suspended in Ringer\/HEPES buffer at pH 7.4 and 4\u00b0C for\n                    <jats:sup>31<\/jats:sup>\n                    P NMR studies. Cell adhesion and growth rate decreased with decreasing pH, but, down to ca pH 6.1, trypan blue exclusion and the observed levels of nucleoside di\u2010 and triphosphates (range: 22\u201337% of total phosphates detected), phosphocreatine (PCr, 2\u20135%) and P\n                    <jats:sub>i<\/jats:sub>\n                    (5\u201311%) did not vary significantly with pH. For XP29MAmal cells in exponential growth phosphocholine levels decreased from 18\u201328% at pH 7.0 to ca 5% at pH 6.0, while phosphoethanolamine levels increased from 2\u20137% to 15%. Glycerophosphocholine (GPC) levels increased from ca 7% at pH 7.2 to 13% at pH 6.3. At pH&lt;6.3 cytidine 5\u2032\u2010diphosphate (CDP) choline became detectable (8\u201316%, \u03b4\n                    <jats:sub>P<\/jats:sub>\n                    : P\n                    <jats:sub>\u03b1<\/jats:sub>\n                    = \u22128.13 ppm, P\n                    <jats:sub>\u03b2<\/jats:sub>\n                    = 8.93 ppm, for PCr = 0 ppm). However, confluent cells did not accumulate CDP\u2010choline when the pH was lowered. The cell lines CX\u20101 and KB also showed the pH effects described herein.\n                  <\/jats:p>","DOI":"10.1002\/nbm.1940030206","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T07:42:24Z","timestamp":1172994144000},"page":"78-89","source":"Crossref","is-referenced-by-count":49,"title":["<sup>31<\/sup>\n                    P NMR studies of cultured human tumor cells. Influence of ph on phospholipid metabolite levels and the detection of cytidine 5\u2032\u2010diphosphate choline"],"prefix":"10.1002","volume":"3","author":[{"given":"A. C.","family":"Kuesel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Graschew","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. E.","family":"Hull","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Lorenz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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