{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:09:49Z","timestamp":1761487789374,"version":"build-2065373602"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,10,21]],"date-time":"2005-10-21T00:00:00Z","timestamp":1129852800000},"content-version":"vor","delay-in-days":4921,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[1992,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cerebral energy metabolism can be measured non\u2010invasively in unanesthetized neonatal rats with <jats:sup>31<\/jats:sup>P NMR spectroscopy. Using this technique, serial changes in high energy phosphates were determined from the right cerebral hemispheres of 7 day postnatal rat pups during a hypoxic\u2010ischemic insult known to produce focal brain injury. During 3 h of hypoxia\u2010ischemia the concentration of ATP dropped to 33\u00b18% of prehypoxic (baseline) levels, phosphocreatine (PCr)\/P<jats:sub>i<\/jats:sub> decreased from 1.5\u00b10.51 to 0.16\u00b10.06, while pH decreased nominally by 0.2 units. After 2.5 h of recovery in air, ATP returned to 75\u00b110% of baseline levels, PCr\/P<jats:sub>i<\/jats:sub> rose to 1.1\u00b10.28, and pH returned to its normal value of 7.16\u00b10.06. This model was used to test the efficacy of the adenosine deaminase inhibitor, 2\u2010deoxycoformycin (DCF) as a potential neuroprotective drug. The data for the drug\u2010 and saline\u2010treated populations were analyzed by integrating ATP and P<jats:sub>i<\/jats:sub>\/PCr levels over specific time intervals, expressing it relative to baseline levels, and modeling it with cubic splines. Pretreatment with 500 \u03bcg\/kg DCF shows a small, but statistically significant, preservation of both ATP and phosphorylation potential during hypoxia and initial recovery. Brain water content (edema) at 42 h recovery was apparently associated with both mean ATP and mean P<jats:sub>i<\/jats:sub>\/PCr in the last 2 h of hypoxia\u2010ischemia. When ATP fell below 70% of baseline, brain edema was evident at 42 h of recovery. This methodology is suitable for extension to human infants.<\/jats:p>","DOI":"10.1002\/nbm.1940050308","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T09:22:18Z","timestamp":1173000138000},"page":"145-153","source":"Crossref","is-referenced-by-count":20,"title":["<sup>31<\/sup>P NMR spectroscopy of perinatal hypoxic\u2010ischemic brain damage: A model to evaluate neuroprotective drugs in immature rats"],"prefix":"10.1002","volume":"5","author":[{"given":"Gerald D.","family":"William","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca L.","family":"Roberts","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel F.","family":"Heitjan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael B.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,21]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"Smith M. 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