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Data obtained from s.c. tumours of varying sizes (44\u2013600 mm<jats:sup>3<\/jats:sup>) have been correlated with the oxygenation status evaluated using O<jats:sub>2<\/jats:sub>\u2010sensitive needle electrodes. \u03b2\u2010NTP\/P<jats:sub>i<\/jats:sub> and phosphocreatine (PCr)\/P<jats:sub>i<\/jats:sub> ratios derived from <jats:sup>31<\/jats:sup>P MRS were positively correlated to the median tissue <jats:italic>p<\/jats:italic>O<jats:sub>2<\/jats:sub> values. pH declined during growth with intracellular acidosis being evident in tumours &gt;350 mm<jats:sup>3<\/jats:sup>. Whereas lactic acid formation greatly contributed to this decline in small\u2010 and medium\u2010sized tumours, ATP hydrolysis and slowing down of the activities of pumps involved in pH<jats:sub>i<\/jats:sub> regulation seem to be major factors responsible for intracellular acidification in bulky tumours. PCr levels decreased at an early growth stage, whilst ATP concentrations dropped in bulky malignancies only, coinciding with a decrease in adenylate energy charge and a substantial rise in the levels of total P<jats:sub>i<\/jats:sub>. MRS observable (mobile) P<jats:sub>i<\/jats:sub> was consistently lower than [P<jats:sub>i<\/jats:sub>] measured in acid extracts. On average, median <jats:italic>p<\/jats:italic>O<jats:sub>2<\/jats:sub> values of ca 10 mmHg represent a critical threshold for energy metabolism. At higher median O<jats:sub>2<\/jats:sub> tensions, levels of ATP, phosphomonoester and total P<jats:sub>i<\/jats:sub> were relatively constant. This coincided with intracellular alkalosis or neutrality and stable adenylate ratios. On average, median <jats:italic>p<\/jats:italic>O<jats:sub>2<\/jats:sub> values &lt; 10 mmHg coincided with intracellular acidosis, ATP depletion, a drop in energy charge and rising P<jats:sub>i<\/jats:sub> levels.<\/jats:p>","DOI":"10.1002\/nbm.1940070305","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T10:22:56Z","timestamp":1173003776000},"page":"128-136","source":"Crossref","is-referenced-by-count":39,"title":["Intracellular acidosis in murine fibrosarcomas coincides with ATP depletion, hypoxia, and high levels of lactate and total P<sub>i<\/sub>"],"prefix":"10.1002","volume":"7","author":[{"given":"P.","family":"Vaupel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Schaefer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Okunieff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,24]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"6449","article-title":"Blood flow, oxygen and nutrient supply, and metabolic microenvironment of human tumors: a review","volume":"49","author":"Vaupel P.","year":"1989","journal-title":"Cancer Res."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1940050505"},{"key":"e_1_2_1_4_2","first-page":"53","volume-title":"Drug Resistance in Oncology","author":"Vaupel P.","year":"1993"},{"key":"e_1_2_1_5_2","first-page":"3316","article-title":"Oxygenation of human tumors: evaluation of tissue oxygen distribution in breast cancers by computerized O2 tension measurements","volume":"51","author":"Vaupel P.","year":"1991","journal-title":"Cancer Res."},{"key":"e_1_2_1_6_2","first-page":"6098","article-title":"Oxygenation of carcinomas of the uterine cervix: evaluation of computerized O2 tension measurements","volume":"51","author":"Hoeckel M.","year":"1991","journal-title":"Cancer Res."},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1940050515"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.2307\/3577798"},{"key":"e_1_2_1_9_2","first-page":"667","article-title":"A simple and inexpensive method for maintaining a defined flora mouse colony","volume":"27","author":"Sedlacek R. 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