{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T16:48:45Z","timestamp":1761929325606,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,10,21]],"date-time":"2005-10-21T00:00:00Z","timestamp":1129852800000},"content-version":"vor","delay-in-days":4007,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[1994,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The applicability of coupled reversed\u2010phase high performance liquid chromatography (HPLC)\u2010NMR spectroscopy for the detection and identification of paracetamol (<jats:italic>N<\/jats:italic>\u2010(4\u2010hydroxyphenyl)acetamide) and its sulfate, glucuronide and <jats:italic>N<\/jats:italic>\u2010acetylcysteinyl metabolites in the unprocessed biological fluids, human urine, rat urine and rat bile, is investigated. Analysis of these samples was performed by gradient HPLC elution and directly coupled 500 MHz <jats:sup>1<\/jats:sup>H NMR spectroscopy detection using a combination of one\u2010 and two\u2010dimensional NMR methods in stopped\u2010flow mode. The stopped\u2010flow approach is demonstrated to be an efficient technique for identification of drug metabolites which have, for example, a UV\u2010chromophore. Stopped\u2010flow HPLC analysis with NMR detection is a viable technique and halting the chromatographic process several times during a run has a negligible effect on the separation and NMR characterization. The post\u2010acquisition data processing method of 'quantified maximum entropy' is shown to provide a means of improving the quality of spectra for minor components, thus aiding NMR resonance assignments.<\/jats:p>","DOI":"10.1002\/nbm.1940070702","type":"journal-article","created":{"date-parts":[[2007,3,4]],"date-time":"2007-03-04T10:35:48Z","timestamp":1173004548000},"page":"295-303","source":"Crossref","is-referenced-by-count":55,"title":["Evaluation of liquid chromatography coupled with high\u2010field <sup>1<\/sup>H NMR spectroscopy for drug metabolite detection and characterization: The identification of paracetamol metabolites in urine and bile"],"prefix":"10.1002","volume":"7","author":[{"given":"M.","family":"Spraul","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hofmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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