{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:40:28Z","timestamp":1761007228855,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,6,7]],"date-time":"2012-06-07T00:00:00Z","timestamp":1339027200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>This study evaluated the effects of hepatic fibrosis on the multiexponential <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> (MET<jats:sub>2<\/jats:sub>) relaxation of <jats:italic>ex vivo<\/jats:italic> murine liver specimens using an 11.7 T MRI. This animal study was approved by the Institutional Animal Care and Use Committee. Eighteen male C57BL\/6 mice were divided into control (<jats:italic>n<\/jats:italic>\u2009=\u20093) and experimental (<jats:italic>n<\/jats:italic>\u2009=\u200915) groups; the latter group was fed a 3,5\u2010dicarbethoxy\u20101,4\u2010dihydrocollidine\u2010supplemented diet to induce hepatic fibrosis. <jats:italic>Ex vivo<\/jats:italic> liver specimens were imaged using an 11.7 T MRI scanner. A multi\u2010echo spin\u2010echo sequence was utilized for subsequent MET<jats:sub>2<\/jats:sub> analysis. Degrees of fibrosis were determined by a pathologist, as well as by digital image analysis. Scatterplot graphs comparing various features of the MET<jats:sub>2<\/jats:sub> signal decay with the degrees of fibrosis were generated, and correlation coefficients were calculated. Two distinct peaks of the MET<jats:sub>2<\/jats:sub> signal decay were identified in all liver specimens: a short <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> component with a geometric mean <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> (GMT<jats:sub>2<\/jats:sub>) approximating 30 ms; and a long <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> component with GMT<jats:sub>2<\/jats:sub> approximating 400 ms. Strong correlation was found between the degree of hepatic fibrosis and the amplitude of the short <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> component, with a higher degrees of fibrosis associated with a lower amplitude. Moderate correlation was also found between hepatic fibrosis and the GMT<jats:sub>2<\/jats:sub> values of the long <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> component, with higher degrees of fibrosis associated with lower GMT<jats:sub>2<\/jats:sub> values. The study of hepatic microenvironments using MET<jats:sub>2<\/jats:sub> analyses offers potential utility in the ongoing development of the noninvasive assessment of hepatic fibrosis using MRI. 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