{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T02:42:52Z","timestamp":1761619372943,"version":"build-2065373602"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,2,17]],"date-time":"2013-02-17T00:00:00Z","timestamp":1361059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>The aims of this study were to characterise the major saturated and unsaturated lipid peaks in histologically normal cervical epithelium and stroma, dysplastic epithelium (low\u2010grade cervical intraepithelial neoplasia, CIN) and cancer\u2010containing tissue samples from patients with cervical cancer using diffusion\u2010weighted <jats:sup>1<\/jats:sup>H high\u2010resolution magic angle spinning MRS, to determine whether mobile lipid resonances (MLRs) distinguish tissue types and to test for a correlation between MLRs and the number of cytoplasmic lipid droplets. Diffusion\u2010weighted spectra of tissue biopsies were acquired using a stimulated echo sequence with bipolar gradients. Major saturated and unsaturated MLRs were identified and multivariate analysis of peak combinations was used to determine the best separation between tissue classes. Lipid droplets were visualised with Nile red staining and fluorescence microscopy. Correlations of saturated lipid resonances (0.9 and 1.3\u2009ppm), polyunsaturated resonances (2.8\u2009ppm), triglycerides (4.3\u2009ppm) and unsaturated resonances (5.3\u2009ppm) with average droplet number (per image) were investigated using a Spearman rank test. A large heterogeneity in lipid content among samples was observed, resulting in no significant differences in MLR intensities of individual peaks between the three tissue classes. Linear discriminant analysis separated \u2018no cancer\u2019 from \u2018cancer\u2019 based on the intensities at 0.9, 1.3, 2.2 and 2.8\u2009ppm [area under the curve (AUC)\u2009=\u20090.939, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001], \u2018low\u2010grade CIN\u2019 from \u2018cancer\u2019 based on the intensities at 0.9, 4.1, 4.3 and 5.3\u2009ppm (AUC\u2009=\u20090.987, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001) and \u2018no cancer\u2019 from \u2018low\u2010grade CIN\u2019 based on intensities at 0.9, 2.2 and 4.3\u2009ppm (AUC\u2009=\u20090.984, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). The distribution of cytoplasmic lipid droplets was nonuniform and was not related to the presence of epithelial or stromal components. On average, there were more droplets visible in low\u2010grade CIN and cancer\u2010containing tissues. Significant correlations between MLR peaks and lipid droplet number were seen for 0.9 (<jats:italic>p<\/jats:italic>\u2009=\u20090.002), 1.3 (<jats:italic>p<\/jats:italic>\u2009=\u20090.003) and 2.8\u2009ppm (<jats:italic>p<\/jats:italic>\u2009=\u20090.018). MLR combinations indicative of average lipid structure efficiently separated tissue classes. Increased lipid resonances correlated with increased numbers of cytoplasmic lipid droplets. Copyright \u00a9 2013 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/nbm.2923","type":"journal-article","created":{"date-parts":[[2013,2,18]],"date-time":"2013-02-18T16:01:49Z","timestamp":1361203309000},"page":"1096-1102","source":"Crossref","is-referenced-by-count":11,"title":["Characterisation of mobile lipid resonances in tissue biopsies from patients with cervical cancer and correlation with cytoplasmic lipid droplets"],"prefix":"10.1002","volume":"26","author":[{"given":"D.","family":"Zietkowski","sequence":"first","affiliation":[{"name":"CRUK and EPSRC Cancer Imaging Centre, The Institute of Cancer Research Royal Marsden NHS Foundation Trust  Sutton Surrey UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. M.","family":"deSouza","sequence":"additional","affiliation":[{"name":"CRUK and EPSRC Cancer Imaging Centre, The Institute of Cancer Research Royal Marsden NHS Foundation Trust  Sutton Surrey UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. L.","family":"Davidson","sequence":"additional","affiliation":[{"name":"CRUK and EPSRC Cancer Imaging Centre, The Institute of Cancer Research Royal Marsden NHS Foundation Trust  Sutton Surrey UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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