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Increased cortical porosity is a major cause of the decreased strength of osteoporotic bone. The majority of cortical pores, however, are below the resolution limit of MRI. Recent work has shown that porosity can be evaluated by MRI\u2010based quantification of bone water. Bi\u2010exponential <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub>* fitting and adiabatic inversion preparation are the two most common methods purported to distinguish bound and pore water in order to quantify matrix density and porosity. To assess the viability of <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub>* bi\u2010component analysis as a method for the quantification of bound and pore water fractions, we applied this method to human cortical bone at 1.5, 3, 7 and 9.4\u2009T, and validated the resulting pool fractions against micro\u2010computed tomography\u2010derived porosity and gravimetrically determined bone densities. We also investigated alternative methods: two\u2010dimensional <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub>\u2013<jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub>* bi\u2010component fitting by incorporation of saturation recovery, one\u2010 and two\u2010dimensional fitting of Carr\u2013Purcell\u2013Meiboom\u2013Gill (CPMG) echo amplitudes, and deuterium inversion recovery. The short\u2010<jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub>* pool fraction was moderately correlated with porosity (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.70) and matrix density (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.63) at 1.5\u2009T, but the strengths of these associations were found to diminish rapidly as the field strength increased, falling below <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.5 at 3\u2009T. The addition of the <jats:italic>T<\/jats:italic><jats:sub>1<\/jats:sub> dimension to bi\u2010component analysis only slightly improved the strengths of these correlations. <jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub>*\u2010based bi\u2010component analysis should therefore be used with caution. The performance of deuterium inversion recovery at 9.4\u2009T was also poor (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.50 vs porosity and <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.46 vs matrix density). The CPMG\u2010derived short\u2010<jats:italic>T<\/jats:italic><jats:sub>2<\/jats:sub> fraction at 9.4\u2009T, however, was highly correlated with porosity (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.87) and matrix density (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.88), confirming the utility of this method for independent validation of bone water pools. 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