{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:36:58Z","timestamp":1789990618327,"version":"4.0.1"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,4,22]],"date-time":"2015-04-22T00:00:00Z","timestamp":1429660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["R01 NS035192"],"award-info":[{"award-number":["R01 NS035192"]}]},{"name":"NIH","award":["R01 NS070815"],"award-info":[{"award-number":["R01 NS070815"]}]},{"name":"CEITEC - Central European Institute of Technology","award":["CZ.1.05\/1.1.00\/02.0068"],"award-info":[{"award-number":["CZ.1.05\/1.1.00\/02.0068"]}]},{"name":"Clinical and Translational Science Award","award":["5KL2TR113"],"award-info":[{"award-number":["5KL2TR113"]}]},{"name":"National Center for Research Resources (NCRR) Biotechnology Research Resource","award":["P41 RR008079"],"award-info":[{"award-number":["P41 RR008079"]}]},{"DOI":"10.13039\/100000070","name":"National Institute of Biomedical Imaging and Bioengineering","doi-asserted-by":"crossref","award":["P41 EB015894"],"award-info":[{"award-number":["P41 EB015894"]}],"id":[{"id":"10.13039\/100000070","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Institutional Center Cores for Advanced Neuroimaging Award","award":["P30 NS076408"],"award-info":[{"award-number":["P30 NS076408"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p>Hippocampal dysfunction is known to be associated with several neurological and neuropsychiatric disorders such as Alzheimer's disease, epilepsy, schizophrenia and depression; therefore, there has been significant clinical interest in studying hippocampal neurochemistry. However, the hippocampus is a challenging region to study using <jats:sup>1<\/jats:sup>H MRS, hence the use of MRS for clinical research in this region has been limited. Our goal was therefore to investigate the feasibility of obtaining high\u2010quality hippocampal spectra that allow reliable quantification of a neurochemical profile and to establish inter\u2010session reproducibility of hippocampal MRS, including reproducibility of voxel placement, spectral quality and neurochemical concentrations. Ten healthy volunteers were scanned in two consecutive sessions using a standard clinical 3\u2009T MR scanner. Neurochemical profiles were obtained with a short\u2010echo (T<jats:sub>E<\/jats:sub>\u2009=\u200928\u2009ms) semi\u2010LASER localization sequence from a relatively small (~4\u2009mL) voxel that covered about 62% of the hippocampal volume as calculated from segmentation of T<jats:sub>1<\/jats:sub>\u2010weighted images. Voxel composition was highly reproducible between sessions, with test\u2013retest coefficients of variation (CVs) of 3.5% and 7.5% for gray and white matter volume fraction, respectively. Excellent signal\u2010to\u2010noise ratio (~54 based on the N\u2010acetylaspartate (NAA) methyl peak in non\u2010apodized spectra) and linewidths (~9\u2009Hz for water) were achieved reproducibly in all subjects. The spectral quality allowed quantification of NAA, total choline, total creatine, myo\u2010inositol and glutamate with high scan\u2013rescan reproducibility (CV\u2009\u2264\u20096%) and quantification precision (Cram\u00e9r\u2013Rao lower bound, CRLB\u2009&lt;\u20099%). Four other metabolites, including glutathione and glucose, were quantified with scan\u2013rescan CV below 20%. Therefore, the highly optimized, short\u2010echo semi\u2010LASER sequence together with FASTMAP shimming substantially improved the reproducibility and number of quantifiable metabolites relative to prior reports. In addition, the between\u2010session variation in metabolite concentrations, as well as CRLB, was lower than the between\u2010subject variation of the concentrations for most metabolites, indicating that the method has the sensitivity to detect inter\u2010individual differences in the healthy brain. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/nbm.3309","type":"journal-article","created":{"date-parts":[[2015,4,22]],"date-time":"2015-04-22T02:42:49Z","timestamp":1429670569000},"page":"685-693","source":"Crossref","is-referenced-by-count":54,"title":["Feasibility and reproducibility of neurochemical profile quantification in the human hippocampus at 3\u2009T"],"prefix":"10.1002","volume":"28","author":[{"given":"Petr","family":"Bedna\u0159\u00edk","sequence":"first","affiliation":[{"name":"Center for Magnetic Resonance Research Department of Radiology, Medical School University of Minnesota  Minneapolis MN USA"},{"name":"Division of Endocrinology and Diabetes Department of Medicine University of Minnesota  Minneapolis MN USA"},{"name":"Multimodal and Functional Neuroimaging Research Group Central European Institute of Technology, CEITEC MU  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Moheet","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Diabetes Department of Medicine University of Minnesota  Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dinesh K.","family":"Deelchand","sequence":"additional","affiliation":[{"name":"Center for Magnetic Resonance Research Department of Radiology, Medical School University of Minnesota  Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uzay E.","family":"Emir","sequence":"additional","affiliation":[{"name":"Center for Magnetic Resonance Research Department of Radiology, Medical School University of Minnesota  Minneapolis MN USA"},{"name":"Oxford Centre for Functional MRI of the Brain (FMRIB) John Radcliffe Hospital University of Oxford  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn E.","family":"Eberly","sequence":"additional","affiliation":[{"name":"Division of Biostatistics School of Public Health University of Minnesota  Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Bare\u0161","sequence":"additional","affiliation":[{"name":"Behavioral and Social Neuroscience Research Group Central European Institute of Technology, CEITEC MU  Brno Czech Republic"},{"name":"First Department of Neurology Masaryk University and St. Anne's Teaching Hospital  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth R.","family":"Seaquist","sequence":"additional","affiliation":[{"name":"Division of Endocrinology and Diabetes Department of Medicine University of Minnesota  Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fclin","family":"\u00d6z","sequence":"additional","affiliation":[{"name":"Center for Magnetic Resonance Research Department of Radiology, Medical School University of Minnesota  Minneapolis MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.27.070203.144130"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-12-2-118"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awf226"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1029"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.1068"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-003-1235-0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1015950108"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2007.12.011"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.13130531"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eplepsyres.2006.06.004"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/schbul\/sbr069"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2594(199901)41:1<204::AID-MRM29>3.0.CO;2-7"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejrad.2011.01.106"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/hbm.20589"},{"issue":"12","key":"e_1_2_8_16_1","first-page":"1294","article-title":"Increased hippocampal glutamate and volumetric deficits in unmedicated patients with schizophrenia","volume":"70","author":"Kraguljac NV","year":"2013","journal-title":"J. Am. Med. Assoc. Psychiatry"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.20138"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.958"},{"key":"e_1_2_8_19_1","first-page":"1739","volume-title":"Proceedings of the 20th Meeting of the International Society for Magnetic Resonance in Medicine","author":"Terpstra M","year":"2012"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1055"},{"key":"e_1_2_8_21_1","first-page":"773","volume-title":"Proceedings of the 15th Meeting of the International Society for Magnetic Resonance Imaging","author":"Kouwe AJ","year":"2007"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.24814"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074638"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22708"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.21302"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.25295"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.20195"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2594(200002)43:2<319::AID-MRM22>3.0.CO;2-1"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2594(199904)41:4<649::AID-MRM2>3.0.CO;2-G"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.20337"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1109\/TSMC.1978.4310039"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2008.12.033"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910140104"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910300604"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.23080"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.24995"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1754"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2009.05.002"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.10112"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22086"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077572"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2009.10.007"},{"key":"e_1_2_8_43_1","first-page":"939","volume-title":"Proceedings of the 18th Meeting of the International Society for Magnetic Resonance in Medicine","author":"Allaili N","year":"2010"}],"container-title":["NMR in Biomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnbm.3309","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnbm.3309","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.3309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T14:26:51Z","timestamp":1760970411000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/nbm.3309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,22]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,6]]}},"alternative-id":["10.1002\/nbm.3309"],"URL":"https:\/\/doi.org\/10.1002\/nbm.3309","archive":["Portico"],"relation":{},"ISSN":["0952-3480","1099-1492"],"issn-type":[{"value":"0952-3480","type":"print"},{"value":"1099-1492","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,22]]}}}