{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:46:56Z","timestamp":1777614416742,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,12]],"date-time":"2017-11-12T00:00:00Z","timestamp":1510444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR000427"],"award-info":[{"award-number":["UL1TR000427"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:p>Hyperpolarized <jats:sup>13<\/jats:sup>C magnetic resonance imaging (MRI) may be used to non\u2010invasively image the transport and chemical conversion of <jats:sup>13<\/jats:sup>C\u2013labeled compounds <jats:italic>in vivo<\/jats:italic>. In this study, we utilize hyperpolarized <jats:sup>13<\/jats:sup>C MRI to evaluate metabolic markers in the kidneys longitudinally in a mouse model of partial unilateral ureteral obstruction (pUUO). Partial obstruction was surgically induced in the left ureter of nine adult mice, leaving the right ureter as a control. <jats:sup>1<\/jats:sup>H and hyperpolarized [1\u2010<jats:sup>13<\/jats:sup>C]pyruvate MRI of the kidneys was performed 2\u00a0days prior to surgery (baseline) and at 3, 7 and 14\u00a0days post\u2010surgery. Images were evaluated for changes in renal pelvis volume, pyruvate, lactate and the lactate to pyruvate ratio. After 14\u00a0days, mice were sacrificed and immunohistological staining of both kidneys for collagen fibrosis (picrosirius red) and macrophage infiltration (F4\/80) was performed. Statistical analysis was performed using a linear mixed effects model. Significant kidney \u00d7 time interaction effects were observed for both lactate and pyruvate, indicating that these markers changed differently between time points for the obstructed and unobstructed kidneys. Both kidneys showed an increase in the lactate to pyruvate ratio after obstruction, suggesting a shift towards glycolytic metabolism. These changes were accompanied by marked hydronephrosis, fibrosis and macrophage infiltration in the obstructed kidney, but not in the unobstructed kidney. Our results show that pUUO is associated with increased pyruvate to lactate metabolism in both kidneys, with injury and inflammation specific to the obstructed kidney. The work also demonstrates the feasibility of the use of hyperpolarized <jats:sup>13<\/jats:sup>C MRI to study metabolism in renal disease.<\/jats:p>","DOI":"10.1002\/nbm.3846","type":"journal-article","created":{"date-parts":[[2017,11,12]],"date-time":"2017-11-12T20:12:41Z","timestamp":1510517561000},"source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of renal metabolic response to partial ureteral obstruction with hyperpolarized <sup>13<\/sup>C MRI"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1989-4505","authenticated-orcid":false,"given":"David J.","family":"Niles","sequence":"first","affiliation":[{"name":"Medical Physics University of Wisconsin\u2013Madison  Madison WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2760-4886","authenticated-orcid":false,"given":"Jeremy W.","family":"Gordon","sequence":"additional","affiliation":[{"name":"Medical Physics University of Wisconsin\u2013Madison  Madison WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gengwen","family":"Huang","sequence":"additional","affiliation":[{"name":"Surgery University of Wisconsin\u2013Madison  Madison WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon","family":"Reese","sequence":"additional","affiliation":[{"name":"Medicine, Nephrology University of Wisconsin\u2013Madison  Madison WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erin B.","family":"Adamson","sequence":"additional","affiliation":[{"name":"Medical Physics University of Wisconsin\u2013Madison  Madison WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arjang","family":"Djamali","sequence":"additional","affiliation":[{"name":"Medicine, Nephrology University of Wisconsin\u2013Madison  Madison WI USA"},{"name":"University of Wisconsin School of Medicine and Public Health  Madison WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5461-0646","authenticated-orcid":false,"given":"Sean B.","family":"Fain","sequence":"additional","affiliation":[{"name":"Medical Physics University of Wisconsin\u2013Madison  Madison WI USA"},{"name":"University of Wisconsin School of Medicine and Public Health  Madison WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,12]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"North American pediatric renal trials and collaborative studies annual report 2014.https:\/\/web.emmes.com\/study\/ped\/index.htm. 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