{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:45:28Z","timestamp":1761007528906,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,20]],"date-time":"2017-12-20T00:00:00Z","timestamp":1513728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006391","name":"Centre d'Imagerie BioM\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006391","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:p>This study demonstrates the suitability of magnetic resonance imaging (MRI) and magnetic resonance angiography (MRA) for the imaging of congenital portosystemic shunts (PSS) in mice, a vascular abnormality in which mesenteric blood bypasses the liver and is instead drained directly to the systemic circulation. The non\u2010invasive diagnosis performed in tandem with other experimental assessments permits further characterization of liver, whole\u2010body and brain metabolic defects associated with PSS. Magnetic resonance measurements were performed in a 26\u2010cm, horizontal\u2010bore, 14.1\u2010T magnet. MRA was obtained with a three\u2010dimensional gradient echo sequence (GRE; in\u2010plane resolution, 234 \u00d7 250 \u00d7 234 \u03bcm<jats:sup>3<\/jats:sup>) using a birdcage coil. Two\u2010dimensional GRE MRI with high spatial resolution (in\u2010plane resolution, 100 \u00d7 130 \u03bcm<jats:sup>2<\/jats:sup>; slices, 30 \u00d7 0.3\u00a0mm) was performed using a surface coil. Brain\u2010 (dorsal hippocampus) and liver\u2010localized <jats:sup>1<\/jats:sup>H magnetic resonance spectroscopy (MRS) was also performed with the surface coil. Whole\u2010body metabolic status was evaluated with an oral glucose tolerance test (OGTT). Both MRA and anatomical MRI allowed the identification of hepatic vessels and the diagnosis of PSS in mice. The incidence of PSS was about 10%. Hepatic lipid content was higher in PSS than in control mice (5.1 \u00b1 2.8% <jats:italic>versus<\/jats:italic> 1.8 \u00b1 0.6%, <jats:italic>p<\/jats:italic> = 0.02). PSS mice had higher brain glutamine concentration than controls (7.3 \u00b1 1.0\u00a0\u03bcmol\/g <jats:italic>versus<\/jats:italic> 2.7 \u00b1 0.6\u00a0\u03bcmol\/g, <jats:italic>p<\/jats:italic> &lt; 0.0001) and, conversely, lower <jats:italic>myo\u2010<\/jats:italic>inositol (4.2 \u00b1 0.6\u00a0\u03bcmol\/g <jats:italic>versus<\/jats:italic> 6.0 \u00b1 0.4\u00a0\u03bcmol\/g, <jats:italic>p<\/jats:italic> &lt; 0.0001), taurine (9.7 \u00b1 1.2\u00a0\u03bcmol\/g <jats:italic>versus<\/jats:italic> 11.0 \u00b1 0.4\u00a0\u03bcmol\/g, <jats:italic>p<\/jats:italic> &lt; 0.01) and total choline (0.9 \u00b1 0.1\u00a0\u03bcmol\/g <jats:italic>versus<\/jats:italic> 1.2 \u00b1 0.1\u00a0\u03bcmol\/g, <jats:italic>p<\/jats:italic> &lt; 0.001) concentrations. Fasting blood glucose and plasma insulin were lower in PSS than in control mice (4.7 \u00b1 0.5mM <jats:italic>versus<\/jats:italic> 8.8 \u00b1 0.6mM, <jats:italic>p<\/jats:italic> &lt; 0.0001; and 0.04 \u00b1 0.03\u00a0\u03bcg\/L <jats:italic>versus<\/jats:italic> 0.3 \u00b1 0.2\u00a0\u03bcg\/L, <jats:italic>p<\/jats:italic> = 0.02, respectively). Glucose clearance during OGTT was delayed and less efficient in PSS mice than in controls. Thus, given the non\u2010negligible incidence of PSS in inbred mice, the undiagnosed presence of PSS will, importantly, have an impact on experimental outcomes, notably in studies addressing brain, liver or whole\u2010body metabolism.<\/jats:p>","DOI":"10.1002\/nbm.3873","type":"journal-article","created":{"date-parts":[[2017,12,20]],"date-time":"2017-12-20T09:34:35Z","timestamp":1513762475000},"source":"Crossref","is-referenced-by-count":7,"title":["Non\u2010invasive diagnosis and metabolic consequences of congenital portosystemic shunts in C57BL\/6\u00a0J mice"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9684-8716","authenticated-orcid":false,"given":"Ana Francisca","family":"Soares","sequence":"first","affiliation":[{"name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne \u2013 Laboratory for Functional and Metabolic Imaging (LIFMET)  Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxia","family":"Lei","sequence":"additional","affiliation":[{"name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Center for Biomedical Imaging (CIBM)  Lausanne Switzerland"},{"name":"University of Geneva Department of Radiology, Faculty of Medicine  Geneva Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2015.09.025"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.sempedsurg.2012.05.006"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2009.02.004"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2011.09.001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-011-9364-0"},{"key":"e_1_2_8_7_1","first-page":"5","article-title":"Congenital portosystemic shunt: our experience","volume":"2015","author":"Timpanaro T","year":"2015","journal-title":"Case Rep Pediatr"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1148\/rg.313105070"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00261-003-0131-x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00247-003-1002-x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1148\/rg.243035046"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069782"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3345"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3761"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","unstructured":"ChengT MagillAW CommentA GruetterR LeiH.Ultra\u2010high field birdcage coils: a comparison study at 14.1 T.36th Annual International Conference of the IEEE Engineering in Medicine and Biology Society Chicago IL USA 2014; 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