{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:46:47Z","timestamp":1761007607536,"version":"build-2065373602"},"reference-count":61,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T00:00:00Z","timestamp":1539734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["320030\u2010138150","CRSII3_147692"],"award-info":[{"award-number":["320030\u2010138150","CRSII3_147692"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:p>Listeria rhombencephalitis is caused by infection with <jats:styled-content style=\"fixed-case\"><jats:italic>Listeria monocytogenes<\/jats:italic><\/jats:styled-content> and is associated with a high mortality rate in humans and ruminants. Little is known about the metabolic changes associated with neurolisteriosis in particular and infectious central nervous system (CNS) diseases in general. The purpose of our study was to investigate the metabolic changes associated with listeria rhombencephalitis in small ruminants (goats and sheep) as a model for inflammatory CNS disease by <jats:sup>1<\/jats:sup>H high\u2010resolution magic angle spinning nuclear magnetic resonance (<jats:sup>1<\/jats:sup>H HR\u2010MAS NMR) spectroscopy of brain biopsies obtained from the brainstem and thalamus. Statistical analysis revealed distinct differences in the metabolic profile of brainstem biopsies, the primary location of listeria rhombencephalitis with moderate or severe inflammatory changes. <jats:italic>N<\/jats:italic>\u2010Acetylaspartate (NAA), <jats:italic>N<\/jats:italic>\u2010acetylaspartylglutamate, choline, myo\u2010inositol and scyllo\u2010inositol were decreased, and glycine, phosphocholine, taurine and lactate were increased, in the diseased group (<jats:italic>n<\/jats:italic>\u00a0=\u00a013) in comparison with the control group (<jats:italic>n<\/jats:italic>\u00a0=\u00a012). In the thalamus, which showed no or only mild inflammatory changes in the majority of animals, no statistically significant metabolic changes were observed. However, trends for metabolic alterations were partly the same as those found in the brainstem, including NAA, choline and lactate. This may be an indicator of metabolic changes occurring in the early stages of the disease. Therefore, further research with a larger number of animals is needed to evaluate the presence of subtle metabolic changes associated with mild inflammatory changes in the thalamus. In conclusion, <jats:sup>1<\/jats:sup>H HR\u2010MAS NMR investigation of listeria rhombencephalitis identified brain metabolite changes, offering new insights into the disease pathophysiology.<\/jats:p>","DOI":"10.1002\/nbm.4023","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T07:38:10Z","timestamp":1539761890000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Metabolic profiling of listeria rhombencephalitis in small ruminants by <sup>1<\/sup>H high\u2010resolution magic angle spinning NMR spectroscopy"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5415-3780","authenticated-orcid":false,"given":"Christina","family":"Precht","sequence":"first","affiliation":[{"name":"Clinical Radiology, Department of Clinical Veterinary Medicine University of Bern  Bern 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