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In DMD, the absence of the dystrophin protein causes decreased sarcolemmal integrity resulting in progressive replacement of muscle with fibrofatty tissue. The effects of lacking dystrophin on muscle and systemic metabolism are still unclear. Therefore, to determine the impact of the absence of dystrophin on metabolism, we investigated the metabolic and lipid profile at two different, well\u2010defined stages of muscle damage and stabilization in <jats:italic>mdx<\/jats:italic> mice. We measured NMR\u2010detectable metabolite and lipid profiles in the serum and muscles of <jats:italic>mdx<\/jats:italic> mice at 6 and 24\u00a0weeks of age. Metabolites were determined in muscle in vivo using <jats:sup>1<\/jats:sup>H MRI\/MRS, in isolated muscles using <jats:sup>1<\/jats:sup>H\u2010HR\u2010MAS NMR, and in serum using high resolution <jats:sup>1<\/jats:sup>H\/<jats:sup>13<\/jats:sup>C NMR. Dystrophic mice were found to have a unique lipid saturation profile compared with control mice, revealing an age\u2010related metabolic change. In the 6\u2010week\u2010old <jats:italic>mdx<\/jats:italic> mice, serum lipids were increased and the degree of lipid saturation changed between 6 and 24\u00a0weeks. The serum taurine\u2010creatine ratio increased over the life span of <jats:italic>mdx,<\/jats:italic> but not in control mice. Furthermore, the saturation index of lipids increased in the serum but decreased in the tissue over time. Finally, we demonstrated associations between MRI\u2010T<jats:sub>2<\/jats:sub>, a strong indicator of inflammation\/edema, with tissue and serum lipid profiles. These results indicate the complex temporal changes of metabolites in the tissue and serum during repetitive bouts of muscle damage and regeneration that occur in dystrophic muscle.<\/jats:p>","DOI":"10.1002\/nbm.4075","type":"journal-article","created":{"date-parts":[[2019,3,8]],"date-time":"2019-03-08T07:18:01Z","timestamp":1552029481000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Age\u2010dependent changes in metabolite profile and lipid saturation in dystrophic mice"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8905-6021","authenticated-orcid":false,"given":"Brittany","family":"Lee\u2010McMullen","sequence":"first","affiliation":[{"name":"Department of Physiology and Functional Genomics University of Florida  Gainesville FL USA"},{"name":"Department of Biochemistry and Molecular Biology, Southeast Center for Integrated Metabolomics University of Florida  Gainesville 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