{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T12:35:22Z","timestamp":1777293322257,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T00:00:00Z","timestamp":1553731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005617","name":"BHF Centre of Research Excellence, Oxford","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005617","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["FS\/14\/54\/30946"],"award-info":[{"award-number":["FS\/14\/54\/30946"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["FS\/15\/80\/31803"],"award-info":[{"award-number":["FS\/15\/80\/31803"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["RG\/13\/8\/30266"],"award-info":[{"award-number":["RG\/13\/8\/30266"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["098436\/Z\/12\/Z"],"award-info":[{"award-number":["098436\/Z\/12\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:p>Changes in the kinetics of the creatine kinase (CK) shuttle are sensitive markers of cardiac energetics but are typically measured at rest and in the prone position. This study aims to measure CK kinetics during pharmacological stress at 3\u00a0T, with measurement in the supine position. A shorter \u201cstressed saturation transfer\u201d (StreST) extension to the triple repetition time saturation transfer (TRiST) method is proposed. We assess scanning in a supine position and validate the MR measurement against biopsy assay of CK activity. We report normal ranges of stress CK forward rate (k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup>) for healthy volunteers and obese patients.<\/jats:p><jats:p>TRiST measures k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup> in 40\u00a0min at 3\u00a0T. StreST extends the previously developed TRiST to also make a further k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup> measurement during &lt;20\u00a0min of dobutamine stress. We test our TRiST implementation in skeletal muscle and myocardium in both prone and supine positions. We evaluate StreST in the myocardium of six healthy volunteers and 34 obese subjects. We validated MR\u2010measured k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup> against biopsy assays of CK activity.<\/jats:p><jats:p>TRiST k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup> values matched literature values in skeletal muscle (k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup>\u00a0=\u00a00.25\u00a0\u00b1\u00a00.03\u00a0s<jats:sup>\u22121<\/jats:sup> vs 0.27\u00a0\u00b1\u00a00.03\u00a0s<jats:sup>\u22121<\/jats:sup>) and myocardium when measured in the prone position (0.32\u00a0\u00b1\u00a00.15\u00a0s<jats:sup>\u22121<\/jats:sup>), but a significant difference was found for TRiST k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup> measured supine (0.24\u00a0\u00b1\u00a00.12\u00a0s<jats:sup>\u22121<\/jats:sup>). This difference was because of different respiratory\u2010 and cardiac\u2010motion\u2010induced B<jats:sub>0<\/jats:sub> changes in the two positions. Using supine TRiST, cardiac k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup> values for normal\u2010weight subjects were 0.15\u00a0\u00b1\u00a00.09\u00a0s<jats:sup>\u22121<\/jats:sup> at rest and 0.17\u00a0\u00b1\u00a00.15\u00a0s<jats:sup>\u22121<\/jats:sup> during stress. For obese subjects, k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup> was 0.16\u00a0\u00b1\u00a00.07\u00a0s<jats:sup>\u22121<\/jats:sup> at rest and 0.17\u00a0\u00b1\u00a00.10\u00a0s<jats:sup>\u22121<\/jats:sup> during stress. Rest myocardial k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup> and CK activity from LV biopsies of the same subjects correlated (<jats:italic>R<\/jats:italic>\u00a0=\u00a00.43, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.03).<\/jats:p><jats:p>We present an independent implementation of TRiST on the Siemens platform using a commercially available coil. Our extended StreST protocol enables cardiac k<jats:sub>f<\/jats:sub><jats:sup>CK<\/jats:sup> to be measured during dobutamine\u2010induced stress in the supine position.<\/jats:p>","DOI":"10.1002\/nbm.4085","type":"journal-article","created":{"date-parts":[[2019,3,28]],"date-time":"2019-03-28T06:47:52Z","timestamp":1553755672000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Localized rest and stress human cardiac creatine kinase reaction kinetics at 3\u00a0T"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7159-7025","authenticated-orcid":false,"given":"William T.","family":"Clarke","sequence":"first","affiliation":[{"name":"Oxford Centre for Clinical Magnetic Resonance Research (OCMR), Division of Cardiovascular Medicine RDM University of Oxford, John Radcliffe Hospital  Oxford UK"},{"name":"Wellcome Centre for Integrative Neuroimaging, FMRIB University of Oxford, John 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