{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:36:53Z","timestamp":1761007013514,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T00:00:00Z","timestamp":1584316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009553","name":"Agentura Pro Zdravotnick\u00fd V\u00fdzkum \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["16\u201027262A"],"award-info":[{"award-number":["16\u201027262A"]}],"id":[{"id":"10.13039\/501100009553","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["IN 00023001"],"award-info":[{"award-number":["IN 00023001"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:p>An unknown intense signal (P<jats:sub>un<\/jats:sub>) with a mean chemical shift of 5.3 ppm was observed in <jats:sup>31<\/jats:sup>P MR spectra from the calf muscles of patients with the diabetic foot syndrome. The aim of the study was to identify the origin of this signal and its potential as a biomarker of muscle injury. Calf muscles of 68 diabetic patients (66.3 \u00b1 8.6 years; body mass index = 28.2 \u00b1 4.3 kg\/m<jats:sup>2<\/jats:sup>) and 12 age\u2010matched healthy controls were examined by (dynamic) <jats:sup>31<\/jats:sup>P MRS (3 T system, <jats:sup>31<\/jats:sup>P\/<jats:sup>1<\/jats:sup>H coil). Phantoms (glucose\u20101\u2010phosphate, P<jats:sub>i<\/jats:sub> and PCr) were measured at pH values of 7.05 and 7.51. At rest, P<jats:sub>un<\/jats:sub> signals with intensities higher than 50% of the P<jats:sub>i<\/jats:sub> intensity were observed in 10 of the 68 examined diabetic subjects. We tested two hypothetical origins of the P<jats:sub>un<\/jats:sub> signal: (1) phosphorus from phosphoesters and (2) phosphorus from extra\u2010 and intracellular alkaline phosphate pools. 2,3\u2010diphosphoglycerate and glucose\u20101\u2010phosphate are the only phosphoesters with signals in the chemical shift region close to 5.3 ppm. Both compounds can be excluded: 2,3\u2010diphosphoglycerate due to the missing second signal component at 6.31 ppm; glucose\u20101\u2010phosphate because its chemical shifts are about 0.2 ppm downfield from the P<jats:sub>i<\/jats:sub> signal (4.9 ppm). If the P<jats:sub>un<\/jats:sub> signal is from phosphate, it represents a pH value of 7.54 \u00b1 0.05. Therefore, it could correspond to signals of P<jats:sub>i<\/jats:sub> in mitochondria. However, patients with critical limb ischemia have rather few mitochondria and so the P<jats:sub>un<\/jats:sub> signal probably originates from interstitia. Our data suggest that the increased P<jats:sub>un<\/jats:sub> signal observed in patients with the diabetic foot syndrome is a biomarker of severe muscular damage.<\/jats:p>","DOI":"10.1002\/nbm.4295","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T23:45:16Z","timestamp":1584402316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Origin of the <sup>31<\/sup>P MR signal at 5.3 ppm in patients with critical limb ischemia"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9216-2810","authenticated-orcid":false,"given":"Petr","family":"Sedivy","sequence":"first","affiliation":[{"name":"MR Unit, Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Dezortova","sequence":"additional","affiliation":[{"name":"MR Unit, Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miloslav","family":"Drobny","sequence":"additional","affiliation":[{"name":"MR Unit, Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michal","family":"Dubsky","sequence":"additional","affiliation":[{"name":"Department of Diabetology Institute for Clinical and Experimental Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tereza","family":"Dusilova","sequence":"additional","affiliation":[{"name":"MR Unit, Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Kovar","sequence":"additional","affiliation":[{"name":"Experimental Medicine Centre Institute for Clinical and Experimental Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Milan","family":"Hajek","sequence":"additional","affiliation":[{"name":"MR Unit, Department of Diagnostic and Interventional Radiology Institute for Clinical and Experimental Medicine  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2017.01.018"},{"issue":"1","key":"e_1_2_7_3_1","first-page":"9","article-title":"P\u201031 magnetic resonance spectroscopy. A tool for diagnostic purposes and pathophysiological insights in muscle diseases","volume":"56","author":"Mattei JP","year":"2004","journal-title":"Reumatismo"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4598(200009)23:9<1316::AID-MUS2>3.0.CO;2-I"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)43389-9"},{"issue":"1","key":"e_1_2_7_6_1","first-page":"43","article-title":"Quantitative interpretation of bioenergetic data from 31P and 1H magnetic resonance spectroscopic studies of skeletal muscle: an analytical review","volume":"10","author":"Kemp GJ","year":"1994","journal-title":"Magn Reson Q"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12307"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.25058"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1725"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2018.00062"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4086"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1517"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep20087"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076628"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3839"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3352"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.2785"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1393976"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1118\/1.4914448"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1088\/0957-0233\/20\/10\/104035"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmre.1997.1244"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910030107"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1192"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.37.1.165"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004424-199711000-00001"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00200.2010"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1081\/JCMR-100108586"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470512968"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.geoderma.2014.12.016"},{"issue":"17","key":"e_1_2_7_30_1","doi-asserted-by":"crossref","first-page":"6172","DOI":"10.1016\/S0021-9258(17)34595-7","article-title":"The determination of the free magnesium level in the human red blood cell by 31P NMR","volume":"253","author":"Gupta RK","year":"1978","journal-title":"J Biol Chem"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004424-199902000-00004"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.2337\/diab.39.2.157"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/118.5.959"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201110767"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00993.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780220813"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1959.14.5.804"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16657095"}],"container-title":["NMR in Biomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnbm.4295","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.4295","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/nbm.4295","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.4295","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T11:15:05Z","timestamp":1760958905000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/nbm.4295"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,16]]},"references-count":37,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1002\/nbm.4295"],"URL":"https:\/\/doi.org\/10.1002\/nbm.4295","archive":["Portico"],"relation":{},"ISSN":["0952-3480","1099-1492"],"issn-type":[{"type":"print","value":"0952-3480"},{"type":"electronic","value":"1099-1492"}],"subject":[],"published":{"date-parts":[[2020,3,16]]},"assertion":[{"value":"2019-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4295"}}