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Given the high contribution of genetic predisposition and the underlying pathophysiology of neurohormonal circuits, it seems that pharmacological targeting of these mechanisms may provide us with better therapeutic outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:sup>1<\/jats:sup>H\u2010NMR spectroscopy was used to measure concentrations of the hypothalamus and brain stem metabolites in an activity\u2010based rodent model (ABA) after subcutaneous administration of kisspeptin\u201010. Because anorexia mainly affects young women and often leads to hypogonadotropic\u2010hypogonadism, we investigated the influence of this neuropeptide, which is involved in reproductive function by regulating the hypothalamic\u2013pituitary\u2010gonadal axis, on the ABA model development.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Kisspeptin reinforced food consumption in an activity\u2010based rodent model of anorexia changing a pattern of weight loss. <jats:sup>1<\/jats:sup>H\u2010NMR spectroscopy of the hypothalamus and brain stem of ABA rats revealed a statistically significant change in the concentration of creatine (Cr; decreased, <jats:italic>P<\/jats:italic> = 0.030), phosphocreatine (PCr; increased, <jats:italic>P<\/jats:italic> = 0.030), \u03b3\u2010aminobutyric acid (GABA; decreased, <jats:italic>P<\/jats:italic> = 0.011), glutathione (GSH; increased, <jats:italic>P<\/jats:italic> = 0.011) and inositol (INS; increased, <jats:italic>P<\/jats:italic> = 0.047) compared to the control group. Subcutaneous administration of kisspeptin reversed the decrease in GABA (<jats:italic>P<\/jats:italic> = 0.018) and Cr (<jats:italic>P<\/jats:italic> = 0.030) levels in the hypothalamus as well as restored glutamate (GLU; <jats:italic>P<\/jats:italic> = 0.040) level in the brain stem.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We suspect that kisspeptin through modulation of hypothalamic GABAergic signaling increases food intake, and thus positively alters brain metabolism.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nbm.4306","type":"journal-article","created":{"date-parts":[[2020,4,6]],"date-time":"2020-04-06T23:03:57Z","timestamp":1586214237000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Hypothalamic and brain stem neurochemical profile in anorectic rats after peripheral administration of kisspeptin\u201010 using <sup>1<\/sup>H\u2010nmr spectroscopy in 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