{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:15:55Z","timestamp":1787235355749,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,7,9]],"date-time":"2020-07-09T00:00:00Z","timestamp":1594252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000098","name":"NIH Clinical Center","doi-asserted-by":"publisher","award":["R01 EB001763"],"award-info":[{"award-number":["R01 EB001763"]}],"id":[{"id":"10.13039\/100000098","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In vivo imaging of oxidative stress can facilitate the understanding and treatment of cardiovascular diseases. We evaluated nitroxide\u2010enhanced MRI with 3\u2010carbamoyl\u2010proxyl (3CP) for the detection of myocardial oxidative stress.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Three mouse models of cardiac oxidative stress were imaged, namely angiotensin II (Ang II) infusion, myocardial infarction (MI), and high\u2010fat high\u2010sucrose (HFHS) diet\u2010induced obesity (DIO). For the Ang II model, mice underwent MRI at baseline and after 7 days of Ang II (\n                      <jats:italic>n<\/jats:italic>\n                      = 8) or saline infusion (\n                      <jats:italic>n<\/jats:italic>\n                      = 8). For the MI model, mice underwent MRI at baseline (\n                      <jats:italic>n<\/jats:italic>\n                      = 10) and at 1 (\n                      <jats:italic>n<\/jats:italic>\n                      = 8), 4 (\n                      <jats:italic>n<\/jats:italic>\n                      = 9), and 21 (\n                      <jats:italic>n<\/jats:italic>\n                      = 8) days after MI. For the HFHS\u2010DIO model, mice underwent MRI at baseline (\n                      <jats:italic>n<\/jats:italic>\n                      = 20) and 18 weeks (\n                      <jats:italic>n<\/jats:italic>\n                      = 13) after diet initiation. The 3CP reduction rate,\n                      <jats:italic>K<\/jats:italic>\n                      <jats:sub>red<\/jats:sub>\n                      , computed using a tracer kinetic model, was used as a metric of oxidative stress. Dihydroethidium (DHE) staining of tissue sections was performed on Day 1 after MI.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      For the Ang II model,\n                      <jats:italic>K<\/jats:italic>\n                      <jats:sub>red<\/jats:sub>\n                      was higher after 7 days of Ang II versus other groups (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). For the MI model,\n                      <jats:italic>K<\/jats:italic>\n                      <jats:sub>red<\/jats:sub>\n                      , in the infarct region was significantly elevated on Days 1 and 4 after MI (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05), whereas\n                      <jats:italic>K<\/jats:italic>\n                      <jats:sub>red<\/jats:sub>\n                      in the noninfarcted region did not change after MI. DHE confirmed elevated oxidative stress in the infarct zone on Day 1 after MI. After 18 weeks of HFHS diet,\n                      <jats:italic>K<\/jats:italic>\n                      <jats:sub>red<\/jats:sub>\n                      was higher in mice after diet versus baseline (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Nitroxide\u2010enhanced MRI noninvasively quantifies tissue oxidative stress as one component of a multiparametric preclinical MRI examination. These methods may facilitate investigations of oxidative stress in cardiovascular disease and related therapies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/nbm.4359","type":"journal-article","created":{"date-parts":[[2020,7,10]],"date-time":"2020-07-10T01:44:55Z","timestamp":1594345495000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Nitroxide\u2010enhanced MRI of cardiovascular oxidative stress"],"prefix":"10.1002","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7622-0603","authenticated-orcid":false,"given":"Soham A.","family":"Shah","sequence":"first","affiliation":[{"name":"Biomedical Engineering University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia X.","family":"Cui","sequence":"additional","affiliation":[{"name":"Biomedical Engineering University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher D.","family":"Waters","sequence":"additional","affiliation":[{"name":"Biomedical Engineering University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soichi","family":"Sano","sequence":"additional","affiliation":[{"name":"Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center University of Virginia  Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center University of Virginia  Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heather","family":"Doviak","sequence":"additional","affiliation":[{"name":"Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center University of Virginia  Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Leor","sequence":"additional","affiliation":[{"name":"Neufield Cardiac Research Institute, Sheba Medical Center, Tel\u2010Aviv University  Tel\u2010Hashomer, Ramat Gan Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth","family":"Walsh","sequence":"additional","affiliation":[{"name":"Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center University of Virginia  Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brent A.","family":"French","sequence":"additional","affiliation":[{"name":"Biomedical Engineering University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick H.","family":"Epstein","sequence":"additional","affiliation":[{"name":"Biomedical Engineering University of Virginia  Charlottesville Virginia USA"},{"name":"Radiology University of Virginia  Charlottesville Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2017.05.035"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.2001.280.6.H2649"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI21625"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehv305"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1667\/RR2668.1"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.60.8.0011"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-2747"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2mb25128k"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2011.05.019"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.25918"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000032100.23772.98"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000096650.91688.28"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.11.020"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF03166686"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics4030442"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.10166"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.12111863"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2005.10.006"},{"issue":"1","key":"e_1_2_10_20_1","first-page":"307","article-title":"Noninvasive imaging of tumor redox status and its modification by tissue glutathione levels","volume":"62","author":"Kuppusamy P","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.20804"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00886.2005"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2303021213"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00705.2011"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22503"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.10210"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.media.2008.06.016"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1109\/TMI.2006.884215"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.acra.2006.02.040"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2010.01.028"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1532-429X-16-18"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200524408"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.02.092"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000254415.31362.a7"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2011.09.018"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2012.03.006"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.02.174"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2015.10.007"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00151.2006"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00060.2012"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.1152"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22280"}],"container-title":["NMR in Biomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnbm.4359","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.4359","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/nbm.4359","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.4359","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T12:25:31Z","timestamp":1760963131000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/nbm.4359"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,9]]},"references-count":42,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1002\/nbm.4359"],"URL":"https:\/\/doi.org\/10.1002\/nbm.4359","archive":["Portico"],"relation":{},"ISSN":["0952-3480","1099-1492"],"issn-type":[{"value":"0952-3480","type":"print"},{"value":"1099-1492","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,7,9]]},"assertion":[{"value":"2019-09-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4359"}}