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Thus, this study aimed to establish and evaluate a robust and fast single voxel 31<\/jats:sup>P MRS method to quantify hepatic \u03b3\u2010ATP concentrations at 11.7 T in three mouse models with different insulin sensitivities and liver fat contents (72\u2010week\u2010old C57BL\/6 control mice, 72\u2010week\u2010old insulin resistant sterol regulatory\u2010element binding protein\u20101c overexpressing (SREBP\u20101c+<\/jats:sup>) mice and 10\u201012\u2010week\u2010old prediabetic non\u2010obese diabetic (NOD) mice). Absolute quantification was performed by employing an external reference and a matching replacement ATP phantom with 3D image selected in vivo spectroscopy 31<\/jats:sup>P MRS. This single voxel 31<\/jats:sup>P MRS method non\u2010invasively quantified hepatic \u03b3\u2010ATP within 17 min and the repeatability tests provided a coefficient of variation of 7.8 \u00b1 1.1%. The mean hepatic \u03b3\u2010ATP concentrations were markedly lower in SREBP\u20101c+<\/jats:sup> mice (1.14 \u00b1 0.10 mM) than in C57BL\/6 mice (2.15 \u00b1 0.13 mM; p<\/jats:italic> < 0.0002) and NOD mice (1.78 \u00b1 0.13 mM; p<\/jats:italic> < 0.006, one\u2010way ANOVA test). In conclusion, this method allows us to rapidly and precisely measure hepatic \u03b3\u2010ATP concentrations, and thereby to non\u2010invasively detect abnormal hepatic energy metabolism in mice with different degrees of insulin resistance and NAFLD. Thus, this 31<\/jats:sup>P MRS will also be useful for future mechanistic as well as therapeutic translational studies in other murine models.<\/jats:p>","DOI":"10.1002\/nbm.4422","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T00:42:37Z","timestamp":1602031357000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In vivo absolute quantification of hepatic \u03b3\u2010ATP concentration in mice using 31<\/sup>P MRS at 11.7 T"],"prefix":"10.1002","volume":"34","author":[{"given":"Maik","family":"Rothe","sequence":"first","affiliation":[{"name":"Institute for Clinical Diabetology German Diabetes Center at Heinrich Heine University, Leibniz Institute for Diabetes Research D\u00fcsseldorf Germany"},{"name":"German Center for Diabetes Research M\u00fcnchen\u2010Neuherberg 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