{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T07:15:11Z","timestamp":1761808511063,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,5,9]],"date-time":"2022-05-09T00:00:00Z","timestamp":1652054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["GA\u2010863037 (BLOC)"],"award-info":[{"award-number":["GA\u2010863037 (BLOC)"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI20\/00658","CIBERDEM"],"award-info":[{"award-number":["PI20\/00658","CIBERDEM"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:p>Liver fibrosis staging is a key element driving the prognosis of patients with chronic liver disease. Currently, biopsy is the only technique capable of diagnosing liver fibrosis in patients with alcohol\u2010related liver disease (ArLD) and nonalcoholic fatty liver disease (NAFLD) unequivocally. Noninvasive (e.g. plasma\u2010based) biomarker assays are attractive tools to diagnose and stage disease, yet must prove that they are reliable and sensitive to be used clinically. Here, we demonstrate proton nuclear magnetic resonance as a method to rapidly quantify the endogenous concentration of ammonium ions from human plasma extracts and show their ability to report upon early and advanced stages of ArLD and NAFLD. We show that, irrespective of the disease etiology, ammonium concentration is a more robust and informative marker of fibrosis stage than current clinically assessed blood hepatic biomarkers. Subject to validation in larger cohorts, the study indicates that the method can provide accurate and rapid staging of ArLD and NAFLD without the need for an invasive biopsy.<\/jats:p>","DOI":"10.1002\/nbm.4745","type":"journal-article","created":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T07:04:23Z","timestamp":1650265463000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Ammonium quantification in human plasma by proton nuclear magnetic resonance for staging of liver fibrosis in alcohol\u2010related liver disease and nonalcoholic fatty liver disease"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5732-5303","authenticated-orcid":false,"given":"Marc","family":"Azagra","sequence":"first","affiliation":[{"name":"Institute for Bioengineering of Catalonia The Barcelona Institute of Science and Technology  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisa","family":"Pose","sequence":"additional","affiliation":[{"name":"Liver Unit, Hospital Clinic, Faculty of Medicine and Health Sciences University of Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"De Chiara","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia The Barcelona Institute of Science and Technology  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martina","family":"Perez","sequence":"additional","affiliation":[{"name":"Liver Unit, Hospital Clinic, Faculty of Medicine and Health Sciences University of Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Avitabile","sequence":"additional","affiliation":[{"name":"Liver Unit, Hospital Clinic, Faculty of Medicine and Health Sciences University of Barcelona  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan\u2010Marc","family":"Servitja","sequence":"additional","affiliation":[{"name":"Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM)  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9896-5024","authenticated-orcid":false,"given":"Laura","family":"Brugnara","sequence":"additional","affiliation":[{"name":"Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer (IDIBAPS), Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Diabetes y Enfermedades Metab\u00f3licas Asociadas (CIBERDEM)  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Ramon\u2010Azc\u00f3n","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia The Barcelona Institute of Science and Technology  Barcelona Spain"},{"name":"ICREA\u2010Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats  Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5076-8526","authenticated-orcid":false,"given":"Irene","family":"Marco\u2010Rius","sequence":"additional","affiliation":[{"name":"Institute for Bioengineering of Catalonia The Barcelona Institute of Science and Technology  Barcelona 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