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However, reference spectra of the healthy spleen are invaluable in studies of splenic malignancies and benign causes of splenomegaly, as well as in the study of its physiology. The purpose of this work was to investigate the feasibility of localized <jats:sup>31<\/jats:sup>P\u2010MRS of healthy spleen in situ in a clinically acceptable measurement time using a clinical 3\u2009T MR scanner. In this work, <jats:sup>31<\/jats:sup>P spectra of five healthy volunteers were measured using single\u2010voxel image\u2010selected in vivo spectroscopy (ISIS). The measurement sequence was augmented by broadband proton decoupling and nuclear Overhauser effect enhancement. It is demonstrated that localized <jats:sup>31<\/jats:sup>P\u2010MRS of the spleen in situ using single\u2010voxel ISIS is feasible on a clinical 3\u2009T scanner in a clinically acceptable acquisition time. However, results have to be corrected for the transmitter excitation profile, and chemical shift displacement errors need to be taken into consideration during placement of the volume of interest. Results presented here could be used as a reference in future studies of splenomegaly caused by haematological malignancies.<\/jats:p>","DOI":"10.1002\/nbm.4779","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T01:58:25Z","timestamp":1654048705000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Phosphorus MRS of healthy human spleen"],"prefix":"10.1002","volume":"35","author":[{"given":"Jan","family":"Weis","sequence":"first","affiliation":[{"name":"Department of Medical Physics Uppsala University Hospital  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maysam","family":"Jafar","sequence":"additional","affiliation":[{"name":"Philips Nordic  Solna Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Per","family":"Liss","sequence":"additional","affiliation":[{"name":"Section of Radiology, Department of Surgical Sciences 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