{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T03:34:49Z","timestamp":1783395289252,"version":"3.54.6"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,11,20]],"date-time":"2022-11-20T00:00:00Z","timestamp":1668902400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The oligomeric amyloid\u2010\u03b2 (oA\u03b2) is a reliable feature for an early diagnosis of Alzheimer's disease (AD). Therefore, the objective of this study was to demonstrate imaging of oA\u03b2 deposits using our developed DNA aptamer called ob5 conjugated with gadolinium (Gd)\u2010dodecane tetraacetic acid (DOTA) as a contrast agent for early diagnosis of AD using MRI. An oA\u03b2\u2010specific aptamer was developed by amide bond formation and conjugated to Gd\u2010DOTA MRI contrast agent and\/or cyanine5 (cy5). We verified the performance of our new contrast agent with an AD mouse model using in vivo and ex vivo fluorescent imaging and animal MRI experiments. The presence of soluble A\u03b2 in 3xTg AD mice was detected using GdDOTA\u2010ob5\u2010cy5 probe ex vivo. Fluorescence intensities of the GdDOTA\u2010ob5\u2010cy5 contrast agent were high in the brains of 3xTg\u2010AD mice, but relatively low in the brains of control mice. The GdDOTA\u2010ob5 contrast agent had higher relaxivity than a clinically available contrast agent. T1\u2010weighted MRI signals in 5\u2010month\u2010old 3xTg AD mice increased at 5\u2009min, were prolonged until 10\u2009min, then decreased 15\u2009min after injecting the GdDOTA\u2010ob5 contrast agent. Our targeted DNA aptamer GdDOTA\u2010ob5 contrast agent could be potentially useful for validating the efficacy of a novel diagnostic contrast agent for selectively targeting neurotoxic oA\u03b2. It could ultimately be used for early diagnosis of AD.<\/jats:p>","DOI":"10.1002\/nbm.4862","type":"journal-article","created":{"date-parts":[[2022,10,29]],"date-time":"2022-10-29T05:13:23Z","timestamp":1667020403000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["An aptamer\u2010based magnetic resonance imaging contrast agent for detecting oligomeric amyloid\u2010\u03b2 in the brain of an Alzheimer's disease mouse model"],"prefix":"10.1002","volume":"36","author":[{"given":"Sang\u2010Tae","family":"Kim","sequence":"first","affiliation":[{"name":"Neuroscience of Lab., Biomedical Research Institute Seoul National University College of Medicine  Seongnam City Geonggido Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyug\u2010Gi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Radiology Kyung Hee University Hospital  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"Neuroscience of Lab., Biomedical Research Institute Seoul National University College of Medicine  Seongnam City Geonggido Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho\u2010Seong","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Surgery Bundang Hospital of Seoul National University  Seongnam City Kyunggeedo Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jee\u2010Hyun","family":"Cho","sequence":"additional","affiliation":[{"name":"Research Equipment Operations Division Korea Basic Science Institute  Cheongju\u2010si Chungcheongbuk\u2010do Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung\u2010Chul","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Pathology Chosun University Medical School  Gwangju Republic of Korea"},{"name":"Department of Education &amp; Research Chosun University Hospital  Gwangju Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taekwan","family":"Lee","sequence":"additional","affiliation":[{"name":"Brain Research Core Facility Korea Brain Research Institute  Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8881-1884","authenticated-orcid":false,"given":"Geon\u2010Ho","family":"Jahng","sequence":"additional","affiliation":[{"name":"Department of Radiology Kyung Hee University Hospital at Gangdong  Seoul Republic of Korea"},{"name":"Department of Medicine, College of Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,20]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.13.4913"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24517"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019442\u2010200201000\u201000004"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.112.117341"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21124477"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12868\u2010016\u20100288\u20101"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.15131"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2010.08.005"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3734"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nicl.2015.10.007"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3cs60086f"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.109.063701"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12084"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddz045"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph14111179"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1041610219002047"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2014.10.016"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201500062"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crad.2014.09.019"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201005285\u20101"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1109\/EMBC44109.2020.9176013"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2020.00021"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.10.2020"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15085742"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/mtna.2014.32"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603838103"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064\u2010020\u201003111\u20101"},{"key":"e_1_2_10_29_1","first-page":"4120","volume-title":"Development of the oligomeric amyloid\u2010beta targeted MRI contrast agents to diagnose the early stage of Alzheimer's disease","author":"Jahng GH","year":"2017"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja101518v"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R075481"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2006.162"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.9b09149"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.12.060"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003\u2010020\u20101016\u2010z"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0cc01413c"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/wnan.1198"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471\u20104159.2008.05769.x"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.752594"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2531\u201015.2016"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4071"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.8b07727"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.201909529"}],"container-title":["NMR in Biomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.4862","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/nbm.4862","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.4862","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T14:31:44Z","timestamp":1760970704000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/nbm.4862"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,20]]},"references-count":42,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/nbm.4862"],"URL":"https:\/\/doi.org\/10.1002\/nbm.4862","archive":["Portico"],"relation":{},"ISSN":["0952-3480","1099-1492"],"issn-type":[{"value":"0952-3480","type":"print"},{"value":"1099-1492","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,20]]},"assertion":[{"value":"2022-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e4862"}}