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Spermine has been considered as an endogenous inhibitor for prostate\u2010confined cancer growth and its expression has shown correlation with prostate cancer growth rates. If established clinically, measurements of spermine bio\u2010synthesis rates in prostates may predict prostate cancer growth and patient outcomes. Using rat models, we tested the feasibility of quantifying spermine bio\u2010synthesis rates with\n                    <jats:sup>13<\/jats:sup>\n                    C NMR. Male Copenhagen rats (10\u00a0weeks, n\u2009=\u20096) were injected with uniformly\n                    <jats:sup>13<\/jats:sup>\n                    C\u2010labeled\n                    <jats:italic>L<\/jats:italic>\n                    \u2010ornithine HCl, and were sacrificed in pairs at 10, 30, and 60\u2009min after injection. Another two rats were injected with saline and sacrificed at 30 min as controls. Prostates were harvested and extracted with perchloric acid and the neutralized solutions were examined by\n                    <jats:sup>13<\/jats:sup>\n                    C NMR at 600\u2009MHz.\n                    <jats:sup>13<\/jats:sup>\n                    C NMR revealed measurable ornithine, as well as putrescine\u2010spermidine\u2010spermine syntheses in rat prostates, allowing polyamine bio\u2010synthetic and ornithine bio\u2010catabolic rates to be calculated. Our study demonstrated the feasibility of\n                    <jats:sup>13<\/jats:sup>\n                    C NMR for measuring bio\u2010synthesis rates of ornithine to spermine enzymatic reactions in rat prostates. The current study established a foundation upon which future investigations of protocols that differentiate prostate cancer growth rates according to the measure of ornithine to spermine bio\u2010synthetic rates may be developed.\n                  <\/jats:p>","DOI":"10.1002\/nbm.4931","type":"journal-article","created":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T12:05:31Z","timestamp":1679313931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<sup>13<\/sup>\n                    C NMR quantification of polyamine syntheses in rat prostate"],"prefix":"10.1002","volume":"36","author":[{"given":"Lindsey A.","family":"Vandergrift","sequence":"first","affiliation":[{"name":"Massachusetts General Hospital and Harvard Medical School  Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanbu","family":"Wang","sequence":"additional","affiliation":[{"name":"Massachusetts 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