{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:14:48Z","timestamp":1788218088633,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003179","name":"Alberta Heritage Foundation for Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003179","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000901","name":"Juvenile Diabetes Research Foundation International","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>For patients with type 1 diabetes mellitus complicated by severe hypoglycemia, clinical islet transplantation is an efficacious alternative to whole pancreas transplantation. While islet transplantation has improved over the last few years, there remain questions regarding its cost\u2010effectiveness and donor allosensitization, which is exacerbated when islets from more than one donor are required. Understanding the features of a pancreas that would provide viable islets prior to isolation may lead to development of an accurate assay that could identify suitable pancreases and provide significant cost savings to a clinical islet transplantation program. In this pilot study, solid\u2010state high\u2010resolution magic angle spinning (HRMAS) nuclear magnetic resonance (NMR) spectroscopy was used to assess samples of convenience of human pancreatic tissue taken prior to islet isolation both before and after incubation using the two\u2010layer perfluorocarbon (PFC)\/University of Wisconsin (UW) solution cold\u2010storage method. We observed that, prior to incubation, human pancreatic tissue exhibited evidence of hypoxia with decreased peak integrals associated with glucose and increased peak integrals corresponding to lactate and free fatty acids. After incubation, we observed a reversal of the hypoxia\u2010induced damage, as integrals corresponding to glucose increased, and those corresponding to lactate and free fatty acid resonances decreased. Interestingly, a significant correlation between the ratio of the glucose integral (at 3.0\u20134.5\u2009ppm) to the sum of the fatty acid (at 0.9\u2009ppm) and lactate + fatty acid (at 1.3\u2009ppm) integrals and glucose responsiveness, a measure of islet viability, of the isolated islets, was observed after incubation in PFC\/UW solution for pancreases that responded to PFC\/UW solution incubation (<jats:italic>p<\/jats:italic>\u2009=\u20090.02). Notably, pancreases with little or no change in the integral ratio after PFC\/UW solution incubation had poor recovery. These results suggest that tissue recovery is a key feature for determining islet cell viability, and further that HRMAS NMR may be a practical method to quickly assess human donor pancreatic tissue prior to islet isolation for clinical transplantation.<\/jats:p>","DOI":"10.1002\/nbm.5207","type":"journal-article","created":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T06:14:25Z","timestamp":1720505665000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["High\u2010resolution magic angle spinning nuclear magnetic resonance of donor pancreatic tissue may predict islet viability prior to isolation"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5100-1594","authenticated-orcid":false,"given":"Carolyn M.","family":"Slupsky","sequence":"first","affiliation":[{"name":"Protein Engineering Network Centres of Excellence, 713 Heritage Medical Research Building University of Alberta  Edmonton Alberta Canada"},{"name":"Department of Nutrition, and Department of Food Science and Technology University of California \u2010 Davis  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian D.","family":"Sykes","sequence":"additional","affiliation":[{"name":"Protein Engineering Network Centres of Excellence, 713 Heritage Medical Research Building University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan R. T.","family":"Lakey","sequence":"additional","affiliation":[{"name":"Surgical\u2010Medical Research Institute, 1074 Dentistry\/Pharmacy Centre University of Alberta  Edmonton Alberta Canada"},{"name":"Department of Surgery and Biomedical Engineering University of California \u2010 Irvine  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(06)68341\u20104"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells12050796"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007273430401"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.21926\/obm.transplant.2004125"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2021.789526"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2018.00202"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4684"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02581331"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118649"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910360502"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2022.105294"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581\u2010022\u201000587\u20108"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890\u2010199105000\u201000044"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890\u2010199209000\u201000036"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890\u2010198809000\u201000027"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890\u2010200212270\u201000007"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890\u2010198804000\u201000001"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/ti.2022.10419"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.01.143"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749\u20106632.2001.tb03826.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4093\/dmj.2019.0181"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890\u2010199004000\u201000008"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/tri.1992.5.s1.391"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12113731"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010021\u201005380\u2010z"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.5630"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/193229680900300616"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.11958"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2018.11.005"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.70714"}],"container-title":["NMR in Biomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.5207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T11:39:52Z","timestamp":1760960392000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/nbm.5207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,9]]},"references-count":30,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/nbm.5207"],"URL":"https:\/\/doi.org\/10.1002\/nbm.5207","archive":["Portico"],"relation":{},"ISSN":["0952-3480","1099-1492"],"issn-type":[{"value":"0952-3480","type":"print"},{"value":"1099-1492","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7,9]]},"assertion":[{"value":"2024-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e5207"}}