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HP [1\u2010<jats:sup>13<\/jats:sup>C] pyruvate and [1\u2010<jats:sup>13<\/jats:sup>C] lactate provide complementary views on the same metabolic pathway, and both have been tested as potential neuroprotective agents in the context of acute brain injuries, with more convincing evidence for a beneficial effect of lactate. Our aim here was to investigate and compare HP [1\u2010<jats:sup>13<\/jats:sup>C] pyruvate and [1\u2010<jats:sup>13<\/jats:sup>C] lactate performance as metabolic contrast agents in the brains of healthy mice and mice subjected to middle cerebral artery occlusion, a model of ischemic stroke. We analyzed the metabolite ratios and quantified the real\u2010time apparent kinetic rates of their cerebral metabolism. We found that the cerebral metabolism of both HP [1\u2010<jats:sup>13<\/jats:sup>C] pyruvate and HP [1\u2010<jats:sup>13<\/jats:sup>C] lactate showed significant alterations after transient cerebral ischemia in mice, reflecting the damage as well as the metabolic reprogramming set in motion to meet the energetic demands in the acute phase of stroke. There was a significant decrease in metabolite ratios (cLPR, cAPR for pyruvate bolus and cPLR, cALR for lactate bolus) and kinetic rates (c<jats:italic>k<\/jats:italic><jats:sub>PL<\/jats:sub> for pyruvate bolus and c<jats:italic>k<\/jats:italic><jats:sub>LP<\/jats:sub> for lactate bolus). These values progressively decreased from sham to 1\u2009h and 2\u2009h after reperfusion measurements. Overall, while pyruvate is better established as an imaging probe, and lactate appears advantageous on the therapeutic side, both bring information to interrogate brain metabolism in physiological and pathophysiological conditions in real time. This study prepares the ground for further investigation to fully exploit the potential of HP metabolic contrasts for stroke theranostics.<\/jats:p>","DOI":"10.1002\/nbm.70094","type":"journal-article","created":{"date-parts":[[2025,7,6]],"date-time":"2025-07-06T23:21:33Z","timestamp":1751844093000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Tracking Hyperpolarized [1\u2010<sup>13<\/sup>C] Pyruvate and [1\u2010<sup>13<\/sup>C] L\u2010Lactate Metabolism in the Healthy and Post\u2010Stroke Mouse Brain"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9615-5642","authenticated-orcid":false,"given":"Thanh\u00a0Phong","family":"L\u00ea","sequence":"first","affiliation":[{"name":"Laboratory of Functional and Metabolic Imaging \u00c9cole polytechnique f\u00e9d\u00e9rale de Lausanne (EPFL)  Lausanne 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