{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T21:30:34Z","timestamp":1784669434781,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T00:00:00Z","timestamp":1762732800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n S\u00e9neca","doi-asserted-by":"publisher","award":["22401\/SF\/23"],"award-info":[{"award-number":["22401\/SF\/23"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["0084906"],"award-info":[{"award-number":["0084906"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hyperpolarized (HP) carbon\u201013 labeled compounds have significantly advanced metabolic research, enabling real\u2010time, non\u2010invasive tracking of metabolic processes in\u00a0vivo and ex\u00a0vivo. These techniques are particularly valuable for studying diseases characterized by altered metabolism, such as cancer, but their high cost and technical complexity limit broader adoption. A critical need exists for cost\u2010effective, high\u2010throughput methods to maximize the utility of these tracers in translational research. In this study, we present a 30\u2010channel microcoil receiver array that enables simultaneous metabolic flux measurements across 30 samples using a single HP dissolution. This system represents a significant increase in throughput compared to previous setups. Using a single dissolution of HP [1\u2010\n                    <jats:sup>13<\/jats:sup>\n                    C]pyruvate, we demonstrate the system's ability to detect significant changes in pyruvate\u2010to\u2010lactate conversion in acute myeloblastic leukemia ML\u20101 cells treated with 2\u2010deoxy\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010glucose. This was achieved with high statistical power (), despite inter\u2010plate variability in perfusion rates of HP pyruvate. This work establishes a new benchmark for high\u2010throughput HP technologies, achieving high statistical power per pyruvate dissolution. By addressing key limitations and optimizing current designs, this technology holds potential in translational metabolic research, particularly in resource\u2010intensive applications such as drug screening and disease modeling. Furthermore, the modular design of the microcoil array is adaptable for a variety of sample types, including tissues, organoids, and patient\u2010derived models. This versatility positions the system as a promising tool for translational research in metabolic diseases, extending beyond oncology to conditions such as diabetes, neurodegeneration, and cardiovascular disorders.\n                  <\/jats:p>","DOI":"10.1002\/nbm.70174","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T04:18:30Z","timestamp":1762834710000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["30\u2010Channel Microcoil System for High\u2010Throughput Metabolic Flux Analysis Using Hyperpolarized\n                    <sup>13<\/sup>\n                    C NMR Spectroscopy"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7390-6071","authenticated-orcid":false,"given":"Nichlas\u00a0Vous","family":"Christensen","sequence":"first","affiliation":[{"name":"The MR Research Centre Aarhus University  Aarhus 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