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High\u2010concentration metabolites can be investigated at 3T using non\u2010edited MRSI, whereas lower\u2010concentration metabolites, such as GABA, typically require specialized editing techniques because of spectral overlap. This study reports on the reproducibility of a protocol containing both co\u2010localized short\u2010TE and GABA\u2010edited multi\u2010slice spin\u2010echo 2D MRSI. Multi\u2010slice, short\u2010TE (TE 20\u2009ms) and GABA\u2010edited (TE 68\u2009ms) MRSI at a nominal spatial resolution of 2.2\u2009cm\n                    <jats:sup>3<\/jats:sup>\n                    was performed twice (7 to 14\u2009days apart) at 3T on 11 healthy volunteers (age range 7 to 43\u2009years). Data analysis was performed in the \u201cOsprey\u201d software package, including retrospective motion compensation, consensus\u2010recommended processing, and linear\u2010combination modeling. Metabolite estimates for six metabolites were quantified relative to total creatine (tCr) and water in 14 regions of interest. Reproducibility was assessed using intra\u2010 and inter\u2010subject coefficients of variation. Short\u2010TE MRSI metabolite estimates for total N\u2010acetylaspartate (tNAA), tCr, total choline (tCho), myo\u2010inositol (mI), and the sum of glutamate and glutamine (Glx) were found to be highly reproducible for both creatine\u2010 and water\u2010referenced concentration estimates, with 77% of the regions of interest meeting the quality\u2010control criteria for both visits and 96% for at least one visit. Average intra\u2010subject CVs were 5.8% and 4.8%, and inter\u2010subject CVs were 11.1% and 9.7% for water\u2010referenced and tCr\u2010referenced estimates, respectively. For GABA+ (GABA\u2009+\u2009macromolecules) estimates, 46% of the voxels of interest met quality\u2010control criteria for both visits, and 82% for at least one visit. In the remaining datasets, the average intra\u2010subject CVs were 13.5% for both quantification methods, and the inter\u2010subject CVs were 13.5% and 16.9% for water\u2010referenced and creatine\u2010referenced estimates, respectively. 3T\u2010MRSI sequences can achieve reproducible mapping with extended brain coverage of five major metabolites (tNAA, tCr, tCho, mI, and Glx). Reproducibility assessment for GABA+ mapping remains challenging, with 18% of the data being rejected in at least one visit, but it yielded acceptable reproducibility in datasets that met quality control criteria in both visits (46%).\n                  <\/jats:p>","DOI":"10.1002\/nbm.70175","type":"journal-article","created":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T04:06:31Z","timestamp":1763697991000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Reproducibility of Metabolic Mapping Using 2D Multi\u2010Slice Short\u2010TE and GABA\u2010Edited Spin\u2010Echo MRSI at 3T in a Single Protocol"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7148-292X","authenticated-orcid":false,"given":"Helge\u00a0J.","family":"Z\u00f6llner","sequence":"first","affiliation":[{"name":"Russell H. Morgan Department of Radiology and Radiological Science The Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dillip\u00a0K.","family":"Senapati","sequence":"additional","affiliation":[{"name":"Russell H. Morgan Department of Radiology and Radiological Science The Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130pek","family":"\u00d6zdemir","sequence":"additional","affiliation":[{"name":"Russell H. 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Morgan Department of Radiology and Radiological Science The Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris\u00a0D.\u00a0M.","family":"Lin","sequence":"additional","affiliation":[{"name":"Russell H. Morgan Department of Radiology and Radiological Science The Johns Hopkins University School of Medicine  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6410-7793","authenticated-orcid":false,"given":"Peter\u00a0B.","family":"Barker","sequence":"additional","affiliation":[{"name":"Russell H. Morgan Department of Radiology and Radiological Science The Johns Hopkins University School of Medicine  Baltimore Maryland USA"},{"name":"F. M. Kirby Research Center for Functional Brain Imaging Kennedy Krieger Institute  Baltimore Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4309"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nic.2010.04.003"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PPO.0000000000000097"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00701\u2010010\u20100833\u20100"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pediatrneurol.2021.07.011"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yebeh.2013.02.018"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1148\/radiology.188.1.8511313"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3174\/ajnr.A1106"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3174\/ajnr.A0810"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.20627"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.20480"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4257"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.27742"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4411"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.NEUROIMAGE.2014.09.032"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.25094"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4368"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2019.116141"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22858"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.21999"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/tomography10040038"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnhum.2020.598435"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.25208"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3898"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3613"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4618"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2020.108827"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22671"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.22324"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022\u20102364(87)90023\u20100"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1214\/ss\/1038425655"},{"key":"e_1_2_12_33_1","unstructured":"H. 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