{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T19:55:10Z","timestamp":1781812510934,"version":"3.54.5"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T00:00:00Z","timestamp":1780185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T00:00:00Z","timestamp":1780185600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Magnetic resonance spectroscopy provides novel noninvasive tools for postmortem forensic investigations. While postmortem temperature assessment using\n                    <jats:sup>1<\/jats:sup>\n                    H\u2010MRS has recently been established, noninvasive determination of brain tissue pH remains largely unexplored despite evidence linking pH to the cause of death. In this study, we investigated the feasibility of in\u00a0situ brain pH determination using postmortem\n                    <jats:sup>1<\/jats:sup>\n                    H\u2010MRS based on the chemical shift difference between myo\u2010inositol (mI, reference metabolite) and acetate (Ace, indicator metabolite) in 58 decedents. LCModel\u2010based fitting strategies were evaluated using simulations that mimic postmortem brain\n                    <jats:sup>1<\/jats:sup>\n                    H\u2010MRS spectra in order to identify the most accurate and precise approach and to assess factors affecting chemical shift determination. The in\u00a0situ MRS data were subsequently analyzed using the optimal strategy. Brain pH values derived from the mI\u2010Ace chemical shift difference were validated against pH measurements obtained from homogenized brain tissue samples. Our results demonstrate that the optimal fitting is achieved by allowing increased flexibility in the chemical shift determination of Ace. Using this approach, robust detection of low pH values in postmortem brain tissue is achieved. These findings demonstrate the feasibility of Ace\u2010based\n                    <jats:sup>1<\/jats:sup>\n                    H\u2010MRS for noninvasive in\u00a0situ assessment of postmortem brain pH and indicate its potential for forensic applications.\n                  <\/jats:p>","DOI":"10.1002\/nbm.70323","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T06:34:21Z","timestamp":1780295661000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Situ pH Determination Using\n                    <sup>1<\/sup>\n                    H\u2010MRS Acetate Chemical Shift for Noninvasive Postmortem Examinations"],"prefix":"10.1002","volume":"39","author":[{"given":"Sabina","family":"Frese","sequence":"first","affiliation":[{"name":"Institute of Forensic Medicine University of Zurich  Zurich Switzerland"},{"name":"High Field MR Center, Department of Biomedical Imaging and Image\u2010Guided Therapy Medical University of Vienna  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic","family":"Gascho","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine University of Zurich  Zurich Switzerland"},{"name":"Private University in the Principality of Liechtenstein (UFL)  Triesen Principality of Liechtenstein"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Thali","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine University of Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Kozerke","sequence":"additional","affiliation":[{"name":"Institute for Biomedical Engineering University and ETH Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9722-7783","authenticated-orcid":false,"given":"Niklaus","family":"Zoelch","sequence":"additional","affiliation":[{"name":"Institute of Forensic Medicine University of Zurich  Zurich Switzerland"},{"name":"Department of Psychiatry, Psychotherapy and Psychosomatics, Psychiatric University Hospital Zurich University of Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,31]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fri.2022.200499"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.4081"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414\u2010019\u201002235\u20106"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00414\u2010017\u20101741\u20100"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-45345-2"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082011"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10334\u2010021\u201000971\u20108"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102990.2008.01003a.x"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2020.12.007"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13041\u2010025\u201001180\u20103"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2006.05.010"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf01251916"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.90.261"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2021.01.026"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.267.1.C195"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910380611"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522\u20102594(200005)43:5<665::AID\u2010MRM8>3.0.CO;2\u20103"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.28442"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910360505"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1201\/b10501"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910290518"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.10294"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.934"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.5171"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1062"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726\u2010011\u20100874\u20106"},{"key":"e_1_2_11_28_1","unstructured":"S.Frese D.Gascho M. 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