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While its molecular and cellular mechanisms have been extensively studied, the in\u00a0vivo metabolic dynamics underlying early regeneration remain incompletely characterized. Hyperpolarized [1\u2010\n                    <jats:sup>13<\/jats:sup>\n                    C]pyruvate MRI (HP\u2010MRI) offers a unique, noninvasive method to assess real\u2010time metabolic fluxes in regenerating liver tissue. Twelve male Wistar rats were randomized to either 70% partial hepatectomy (PH;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) or nonsurgical controls (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096). On postoperative day 1, all animals underwent HP\u2010MRI and multiparametric proton MRI. Metabolic fluxes were quantified using area\u2010under\u2010the\u2010curve ratios for lactate\u2010to\u2010pyruvate (L\/P), alanine\u2010to\u2010pyruvate (A\/P), and lactate\u2010to\u2010alanine (L\/A). The PH group showed significantly higher L\/P (0.267 [95% CI: 0.225\u20130.310] vs. 0.168 [95% CI: 0.135\u20130.200];\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001) and A\/P (0.236 [95% CI: 0.153\u20130.319] vs. 0.150 [95% CI: 0.128\u20130.172];\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.028) ratios compared to controls, indicating increased exchange of pyruvate to lactate and alanine. L\/A ratios remained unchanged. These findings were supported by elevated biochemical markers of hepatic injury and changes in quantitative MRI parameters, including reduced ADC and IVIM flow fraction. HP\u2010MRI revealed increased glycolytic and transaminase activity during early liver regeneration, consistent with the metabolic demands of hepatocyte proliferation. These results demonstrate the feasibility of HP\u2010MRI for noninvasive metabolic assessment of liver regeneration in\u00a0vivo and additionally provide insights into early regenerative metabolism. This suggests HP\u2010MRI as a promising tool for assessing postoperative recovery in patients undergoing major hepatectomy.\n                  <\/jats:p>","DOI":"10.1002\/nbm.70340","type":"journal-article","created":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T06:02:10Z","timestamp":1782108130000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hyperpolarized MRI Detects Increased [1\u2010\n                    <sup>13<\/sup>\n                    C]Pyruvate Metabolism in Early Liver Regeneration"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9188-2162","authenticated-orcid":false,"given":"Uffe","family":"Kj\u00e6rgaard","sequence":"first","affiliation":[{"name":"The MR Research Centre, Department of Clinical Medicine Aarhus University  Aarhus Denmark"},{"name":"Department of Surgery Horsens Regional Hospital  Horsens Denmark"},{"name":"Department of Surgery, Section 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