{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:48:22Z","timestamp":1792054102222,"version":"4.4.0"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:00:00Z","timestamp":1791244800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003554","name":"Lundbeckfonden","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003554","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hyperpolarized (HP)\n                    <jats:sup>13<\/jats:sup>\n                    C MRI enables noninvasive assessment of myocardial metabolism. Current approaches typically acquire data only during diastole, ignoring phase\u2010dependent metabolic changes. This study aimed to develop an imaging technique to capture HP [\n                    <jats:sup>13<\/jats:sup>\n                    C]bicarbonate production across both systole and diastole in healthy porcine hearts under rest and stress conditions. Six Danish domestic pigs underwent conventional cardiac MRI and HP [1\u2010\n                    <jats:sup>13<\/jats:sup>\n                    C]pyruvate MRI on an MR750 3\u2009T system (GE HealthCare) before and during pharmacological stress by injecting adenosine (250\u2009\u03bcg\/min\/kg) and dobutamine (5\u2009\u03bcg\/min\/kg). Metabolic imaging was obtained by single\u2010frequency imaging on [\n                    <jats:sup>13<\/jats:sup>\n                    C]bicarbonate. Concurrent off\u2010resonance excitation on [1\u2010\n                    <jats:sup>13<\/jats:sup>\n                    C]pyruvate was utilized to generate a simultaneous pyruvate input function. Bicarbonate\u2010to\u2010pyruvate ratios (Bic\/Pyr ratio) were compared from rest to stress throughout the cardiac cycle. Dynamic metabolic changes were successfully captured with sufficient temporal resolution to resolve both systolic and diastolic phases. From rest to stress, during systole the Bic\/Pyr ratio increased by 150% (95% CI 50%\u2013330%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0076) and during diastole it increased by 170% (95% CI 50%\u2013380%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0083). During rest, the Bic\/Pyr ratio decreased by 21% from systole to diastole (95% CI 4%\u201335%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.027), and during stress, it decreased by 16% (95% CI 4%\u201326%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.019). Simultaneous phase\u2010resolved imaging of [1\u2010\n                    <jats:sup>13<\/jats:sup>\n                    C]pyruvate and [\n                    <jats:sup>13<\/jats:sup>\n                    C]bicarbonate enables high\u2010temporal\u2010resolution assessment of myocardial metabolism during both systole and diastole compared to conventional single\u2010frequency acquisitions. This simultaneous acquisition strategy removes the need for separate pyruvate and bicarbonate acquisitions, doubling the temporal resolution of the resulting image series, while revealing dynamic increases in Bic\/Pyr ratio from rest to stress and between systole and diastole. This approach overcomes the limitations of single\u2010phase imaging and provides a foundation for investigating phase\u2010dependent metabolic alterations in disease.\n                  <\/jats:p>","DOI":"10.1002\/nbm.70422","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T04:47:56Z","timestamp":1791348476000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Continuous Imaging of the Cardiac Cycle Metabolism With Hyperpolarized [1\u2010\n                    <sup>13<\/sup>\n                    C]Pyruvate"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8972-4820","authenticated-orcid":false,"given":"Mohsen","family":"Redda","sequence":"first","affiliation":[{"name":"The MR Research Centre, Department of Clinical Medicine Aarhus University  Aarhus Denmark"},{"name":"Department of Surgery Randers Regional Hospital  Randers Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolaj","family":"B\u00f8gh","sequence":"additional","affiliation":[{"name":"The MR Research Centre, Department of Clinical Medicine Aarhus University  Aarhus Denmark"},{"name":"Department of Surgery and Intensive Care Viborg Regional Hospital  Viborg Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5512-9870","authenticated-orcid":false,"given":"Esben S\u00f8vs\u00f8 Szocska","family":"Hansen","sequence":"additional","affiliation":[{"name":"The MR Research Centre, Department of Clinical Medicine Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9188-2162","authenticated-orcid":false,"given":"Uffe","family":"Kj\u00e6rgaard","sequence":"additional","affiliation":[{"name":"The MR Research Centre, Department of Clinical Medicine Aarhus University  Aarhus Denmark"},{"name":"Department of Surgery Horsens Regional Hospital  Horsens Denmark"},{"name":"Department of Surgery Aarhus University Hospital  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf F.","family":"Schulte","sequence":"additional","affiliation":[{"name":"GE HealthCare  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0317-2911","authenticated-orcid":false,"given":"Christoffer","family":"Laustsen","sequence":"additional","affiliation":[{"name":"The MR Research Centre, Department of Clinical Medicine Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurjhf\/hfr173"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.702795"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cjca.2017.03.009"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2018.00068"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcm.2017.07.001"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacbts.2022.03.017"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/a:1015320423577"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jocmr.2024.101095"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2020.12.023"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.309769"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.28421"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12968\u2010022\u201000860\u20106"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.28796"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.29042"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.317970"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.28691"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.119.316260"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.954388"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/RLI.0000000000001094"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1733835100"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.29259"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.24353"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.28238"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.29478"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12968\u2010023\u201000972\u20107"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00404.2004"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.6.3654"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s003950170006"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1983.245.2.H354"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010013\u20101539\u20102"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071996"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1280161"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108434200"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010021\u201003984\u20102"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1023\/a:1005556227425"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c160016"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3565(24)37317-3"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0033\u20100620(89)90015\u20107"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3565(25)32823-5"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.36.1.185"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/chir.20874"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.33.4.412"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/10742484211048762"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(63)91500\u20109"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.3302"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201065098\u20107"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706235104"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10554\u2010022\u201002661\u20105"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.70182"}],"container-title":["NMR in Biomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.70422","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/nbm.70422","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.70422","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T08:21:21Z","timestamp":1792052481000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/nbm.70422"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,6]]},"references-count":49,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1002\/nbm.70422"],"URL":"https:\/\/doi.org\/10.1002\/nbm.70422","archive":["Portico"],"relation":{},"ISSN":["0952-3480","1099-1492"],"issn-type":[{"value":"0952-3480","type":"print"},{"value":"1099-1492","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,6]]},"assertion":[{"value":"2026-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70422"}}