{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T12:55:25Z","timestamp":1761742525227,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,3,9]],"date-time":"2005-03-09T00:00:00Z","timestamp":1110326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2005,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The utility of <jats:sup>1<\/jats:sup>H NMR spectroscopy is suggested and demonstrated for the diagnosis of <jats:italic>Pseudomonas aeruginosa<\/jats:italic> in urinary tract infection (UTI). The specific property of <jats:italic>P. aeruginosa<\/jats:italic> of metabolizing nicotinic acid to 6\u2010hydroxynicotinic acid (6\u2010OHNA) is exploited. The quantity of 6\u2010OHNA produced correlates well with the viable bacterial count. Other common bacteria causing UTI such as <jats:italic>Escherichia coli, Klebsiella pneumonia, Enterobacter aerogenes, Acinetobacter baumanii, Proteus mirabilis, Citrobacter frundii, Enterococcus faecalis, Streptococcus gp B<\/jats:italic> and <jats:italic>Staphylococcus aureus<\/jats:italic> do not metabolize nicotinic acid under similar conditions. The method provides a single\u2010step documentation of <jats:italic>P. aeruginosa<\/jats:italic> qualitatively as well as quantitatively. The NMR method is demonstrated on urine samples from 30 patients with UTI caused by <jats:italic>P. aeruginosa<\/jats:italic>. 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