{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T11:40:51Z","timestamp":1775130051087,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,8,1]],"date-time":"2005-08-01T00:00:00Z","timestamp":1122854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["NMR in Biomedicine"],"published-print":{"date-parts":[[2005,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Choline and the related compounds phosphocholine (PC) and glycerophosphocholine (GPC) are considered to be important metabolites in oncology. Past studies have demonstrated correlations linking the relative ratios and concentrations of these metabolites with the development and progression of cancer. Currently, <jats:italic>in vivo<\/jats:italic> and tissue <jats:italic>ex vivo<\/jats:italic> magnetic resonance spectroscopy methods have mostly centered on measuring the total concentration of these metabolites and have difficulty in differentiating between them. Here, a new scheme that uses <jats:sup>31<\/jats:sup>P edited <jats:sup>1<\/jats:sup>H spectroscopy to quantify the concentrations of choline, PC and GPC in biological samples is reported and its applicability is demonstrated using samples of human brain tumor extracts. This method is particularly well\u2010suited for analytical situations where the PC and GPC resonances are not sufficiently resolved and\/or are obscured by other metabolites. Consequently, this scheme has the potential to be used for the analysis of choline compounds in <jats:italic>ex vivo<\/jats:italic> tissue samples. Copyright \u00a9 2005 by John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/nbm.973","type":"journal-article","created":{"date-parts":[[2005,8,2]],"date-time":"2005-08-02T21:29:39Z","timestamp":1123018179000},"page":"413-420","source":"Crossref","is-referenced-by-count":36,"title":["Quantification of phosphocholine and glycerophosphocholine with <sup>31<\/sup>P edited <sup>1<\/sup>H NMR spectroscopy"],"prefix":"10.1002","volume":"18","author":[{"given":"Nikolaus M.","family":"Loening","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne M.","family":"Chamberlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea G.","family":"Zepeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. Gilberto","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leo L.","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004310050050"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1492(199911)12:7<413::AID-NBM587>3.0.CO;2-U"},{"issue":"1","key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1177\/135965350300800103","article-title":"Persistent brain abnormalities in antiretroviral\u2010naive HIV patients 3 months after HAART","volume":"8","author":"Chang L","year":"2003","journal-title":"Antivir. Ther."},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0887-8994(02)00491-5"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.schbul.a006942"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1159\/000064927"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-003-0995-2"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-200302100-00013"},{"issue":"4","key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1016\/S1052-5149(25)00674-4","article-title":"Proton MR spectroscopy in multiple sclerosis","volume":"10","author":"Arnold DL","year":"2000","journal-title":"Neuroimag. Clin. N. Am."},{"issue":"5","key":"e_1_2_1_11_2","first-page":"882","article-title":"Developmental delay in children: assessment with proton MR spectroscopy","volume":"23","author":"Filippi CG","year":"2002","journal-title":"AJNR Am. J. Neuroradiol."},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiol.2263011937"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2003.98.2.0269"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.807"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6600704"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.10367"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/hed.10125"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/94.16.1197"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.1999.tb00764.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004424-199903000-00013"},{"issue":"6","key":"e_1_2_1_21_2","first-page":"1890","article-title":"Melanoma tumors acquire a new phospholipid metabolism phenotype under cystemustine as revealed by high\u2010resolution magic angle spinning proton nuclear magnetic resonance spectroscopy of intact tumor samples","volume":"62","author":"Morvan D","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1157"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1099-1492(200005)13:3<129::AID-NBM619>3.0.CO;2-V"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.12.6408"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.775"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmrb.1996.0174"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1492(199805)11:3<93::AID-NBM520>3.0.CO;2-H"},{"issue":"6","key":"e_1_2_1_28_2","first-page":"1890","article-title":"Melanoma tumors acquire a new phospholipid metabolism phenotype under cystemustine as revealed by high\u2010resolution magic angle spinning proton nuclear magnetic resonance spectroscopy of intact tumor samples","volume":"62","author":"Morvan D","year":"2002","journal-title":"Cancer Res."},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.10368"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja00497a058"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmra.1993.1268"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2364(83)90035-5"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/nbm.740"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910240113"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.3171\/jns.1994.81.3.0443"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1492(199701)10:1<2::AID-NBM442>3.0.CO;2-N"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/bcr268"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1492(1998110)11:7<314::AID-NBM522>3.0.CO;2-Z"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2594(199905)41:5<897::AID-MRM7>3.0.CO;2-T"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306209200"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.10379"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1080\/00268979100102191"}],"container-title":["NMR in Biomedicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fnbm.973","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/nbm.973","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T12:20:41Z","timestamp":1760962841000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/nbm.973"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,8]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2005,11]]}},"alternative-id":["10.1002\/nbm.973"],"URL":"https:\/\/doi.org\/10.1002\/nbm.973","archive":["Portico"],"relation":{},"ISSN":["0952-3480","1099-1492"],"issn-type":[{"value":"0952-3480","type":"print"},{"value":"1099-1492","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,8]]}}}