{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T05:51:30Z","timestamp":1791697890390,"version":"4.3.4"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,4,13]],"date-time":"2025-04-13T00:00:00Z","timestamp":1744502400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20 GM135007\u201004S1"],"award-info":[{"award-number":["P20 GM135007\u201004S1"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01 NS093289"],"award-info":[{"award-number":["R01 NS093289"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Neuroprotection"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pre\u2010eclampsia (PE) is a serious hypertensive disorder of pregnancy with lifelong deleterious effects including an increased risk of stroke postpartum (PP). In the present study, we aimed to determine whether previous PE exacerbates ischemic injury during PP and investigate the underlying mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Female Sprague\u2010Dawley rats were studied at 4\u20139 months PP after either a normal pregnancy postpartum (NormP\u2010PP, <jats:italic>n<\/jats:italic>\u2009=\u20097) or experimental PE (ePE) induced using a high\u2010cholesterol diet (ePE\u2010PP, <jats:italic>n<\/jats:italic>\u2009=\u20099). The animals underwent transient middle cerebral artery occlusion for 2\u2009h with 1\u2009h of reperfusion. Dual\u2010site laser Doppler flowmetry was used to measure cerebral blood flow changes in the middle cerebral artery and collateral territories. Ischemic injury was measured using 2,3,5\u2010triphenyl tetrazolium chloride staining. Circulating 8\u2010isoprostane, 3\u2010nitrotyrosine, and oxidized low\u2010density lipoprotein levels were measured using enzyme\u2010linked immunosorbent assays. In separate groups of animals, NormP\u2010PP (<jats:italic>n<\/jats:italic>\u2009=\u200910) and ePE\u2010PP (<jats:italic>n<\/jats:italic>\u2009=\u20099) at 3\u20134 months PP, isolated pial collateral vessels, leptomeningeal anastomoses, and mesenteric arteries were studied using pressure myography.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Previous ePE worsened stroke outcome in the PP period, significantly increasing infarction and edema in ePE\u2010PP compared to NormP\u2010PP animals (40.6\u2009\u00b1\u20097.6% vs. 13.7\u2009\u00b1\u20096.5%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01; 5.1\u2009\u00b1\u20092.0% vs. 2.6\u2009\u00b1\u20090.4%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) despite comparable changes in cerebral blood flow in the middle cerebral artery and pial collateral territories during ischemia and reperfusion. When infarction was analyzed as a function of perfusion deficit, the ePE\u2010PP animals showed greater sensitivity to ischemia. Pial collaterals had increased pressure\u2010induced myogenic tone in ePE\u2010PP compared to NormP\u2010PP rats. Percentage tone at 80\u2009mmHg for ePE\u2010PP vs. NormP\u2010PP was 15.5\u2009\u00b1\u20091.6% vs. 8.6\u2009\u00b1\u20091.9% (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). ePE\u2010PP animals showed significantly elevated levels of circulating 8\u2010isoprostane and 3\u2010nitrotyrosine but not oxidized low\u2010density lipoprotein after transient middle cerebral artery occlusion (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05, and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Worsened stroke outcomes after ePE pregnancy were related to increased ischemia sensitivity, increased pial collateral tone, and elevated levels of oxidative stress markers. Thus, the pathological effects of ePE persisted PP and negatively affected stroke outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/nep3.70002","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T05:10:31Z","timestamp":1744607431000},"page":"172-182","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["History of pre\u2010eclampsia negatively impacts stroke severity postpartum in rats"],"prefix":"10.1002","volume":"3","author":[{"given":"Ari","family":"Kropf","sequence":"first","affiliation":[{"name":"Department of Neurological Sciences University of Vermont Larner College of Medicine Burlington VT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Neurological Sciences University of Vermont Larner College of Medicine Burlington VT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Esposito","sequence":"additional","affiliation":[{"name":"Department of Neurological Sciences University of Vermont Larner College of Medicine Burlington VT USA"},{"name":"Department of Biology, Ecology and Earth Science University of Calabria Rende Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah M.","family":"Tremble","sequence":"additional","affiliation":[{"name":"Department of Neurological Sciences University of Vermont Larner College of Medicine Burlington VT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9172-5941","authenticated-orcid":false,"given":"Marilyn J.","family":"Cipolla","sequence":"additional","affiliation":[{"name":"Department of Neurological Sciences University of Vermont Larner College of Medicine Burlington VT USA"},{"name":"Department of Obstetrics, Gynecology and Reproductive Sciences University of Vermont Larner College of Medicine Burlington VT USA"},{"name":"Department of Pharmacology University of Vermont Larner College of Medicine Burlington VT USA"},{"name":"Department of Electrical and Biomedical Engineering University of Vermont College of Engineering and Mathematical Sciences Burlington VT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,13]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2012.05.006"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajogmf.2023.100864"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12154999"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.120.051460"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/STROKEAHA.123.043052"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11883-019-0798-2"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.4061\/2011\/858134"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2018.06.006"},{"issue":"12","key":"e_1_2_10_10_1","article-title":"The risk of persistent hypertension and chronic kidney disease in early\u2010 and late\u2010onset preeclampsia: A report from developing country","volume":"15","author":"Ernawati E","year":"2023","journal-title":"Cureus"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2015.06.002"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11906-020-01058-w"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/704146"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.122.19408"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preghy.2013.03.004"},{"issue":"1","key":"e_1_2_10_16_1","first-page":"19","article-title":"Change in brain size during and after pregnancy: study in healthy women and women with preeclampsia","volume":"23","author":"Oatridge A","year":"2002","journal-title":"AJNR Am J Neuroradiol"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCOUTCOMES.115.002461"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000003765"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2021.5077"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.285.12.1607"},{"issue":"4","key":"e_1_2_10_21_1","first-page":"309","article-title":"Endothelial dysfunction. 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