{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T16:18:35Z","timestamp":1772813915287,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,4,25]],"date-time":"2002-04-25T00:00:00Z","timestamp":1019692800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurobiol."],"published-print":{"date-parts":[[2002,6,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The neural cell adhesion molecule (CAM) L1 is a member of the immunoglobulin superfamily that has been implicated in neuronal adhesion, neurite outgrowth, and axon guidance. The clinical importance of L1 is illustrated by pathological mutations that lead to hydrocephalus, mental retardation, motor defects, and early mortality. The L1 gene is composed of 28 exons, including exons 2 and 27 that are spliced alternatively, and mutations in exon 2 are associated with severe neurological abnormalities in humans. To elucidate the role of L1 exon 2, a recombinant Fc fusion protein called \u03942L1 was constructed lacking the second exon in the extracellular domain of L1. When bound to fluorescent beads, L1 exhibited homophilic binding while \u03942L1 did not. However, L1 beads coaggregated with the \u03942L1 beads. Similarly, in cell binding studies, L1 bound to L1 and \u03942L1 did not bind to \u03942L1 but it bound moderately to L1. Given the reduced binding of \u03942L1, we tested its effect on neurons. By comparison to L1, a lower percentage of dissociated neurons extended neurites on \u03942L1, and there was a modest decrease in the length of the neurites that grew. Neurite outgrowth from reaggregated neurons was much less robust on \u03942L1 than on L1. The combined results indicate that \u03942L1 does not bind homophilically but it can interact with L1 containing exon 2. The reduced binding and neurite promoting activity of \u03942L1 provides an explanation for certain pathological mutations in L1 that lead to clinically apparent disease in the absence of the normal form of L1 in the nervous system. \u00a9 2002 Wiley Periodicals, Inc. J Neurobiol 51: 237\u2013247, 2002<\/jats:p>","DOI":"10.1002\/neu.10052","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:45:33Z","timestamp":1030319133000},"page":"177-189","source":"Crossref","is-referenced-by-count":43,"title":["L1 mediated homophilic binding and neurite outgrowth are modulated by alternative splicing of exon 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