{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T11:10:13Z","timestamp":1699787413372},"reference-count":8,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":12337,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurobiol."],"published-print":{"date-parts":[[1971,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The energy, ionic, and pH dependence of the influx of labelled <jats:sc>L<\/jats:sc>\u2010glutamic acid has been studied in frog sciatic nerve<\/jats:p><jats:p><jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The addition of 10<jats:sup>\u22122<\/jats:sup> M <jats:sc>D<\/jats:sc>\u2010glucose to the incubation medium was found to inhibit the total influx of glutamic acid from a 10<jats:sup>\u22126<\/jats:sup> M solution by about 40%.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The rate of influx was found to be sensitive to the temperature of the bathing medium. Influx was found to increase with temperature in a linear manner from 10\u00b0C to 34\u00b0C; above 34\u00b0C, the rate of influx dropped with increased temperature.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>At room temperature, the effect of metabolic inhibitors on total influx ranged from 18% inhibition with aresenate to 62% with DNP. Increasing the incubation temperature to 34\u00b0C increased the percentage inhibition, a maximum inhibition of 86% occurring with DNP.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>The cardiac glycoside ouabain (10<jats:sup>\u22123<\/jats:sup> M) slightly inhibited influx (15%).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Reduction of the sodium concentration to 2.3 mM resulted in an 82% decrease in total influx. Omission of potassium or a tenfold increase in potassium concentration had no significant effect on influx. Omission of calcium resulted in a slight stimulation of uptake (16%); a tenfold increase in calcium concentration was slightly inhibitory (15%).<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>No relation was found between influx and the pH of the incubation medium. Lowering the pH to 5.4 or raising it to 9.4 had no significant effect on influx.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>It is concluded that <jats:sc>L<\/jats:sc>\u2010glutamic acid influx in frog sciatic nerve is probably associated with an energy\u2010dependent process.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p>","DOI":"10.1002\/neu.480020210","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T08:50:44Z","timestamp":1104569444000},"page":"181-190","source":"Crossref","is-referenced-by-count":16,"title":["Influx of glutamic acid in peripheral nerve. Energy, ionic, and pH dependence"],"prefix":"10.1002","volume":"2","author":[{"given":"Darrell D.","family":"Wheeler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. L.","family":"Boyarsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"701","DOI":"10.1152\/ajplegacy.1966.210.4.701","article-title":"Some effects of hexoses on the absorption of amino acids","volume":"210","author":"Annegers J. H.","year":"1966","journal-title":"Am. J. 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