{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:59:49Z","timestamp":1758279589150},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":11241,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurobiol."],"published-print":{"date-parts":[[1974,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A highly toxic protein has been isolated from the venom of the snake <jats:italic>Bungarus multicinctus.<\/jats:italic> No major contaminants were detected on isoelectric focusing, gel filtration, or gel electrophoresis. The molecular weight calculated from its amino acid composition (21,800) agreed well with that calculated from gel filtration and equilibrium sedimentation data. Gel electrophoresis showed that the toxin consisted of two subunits of molecular weight 8,800 and 12,400 held together by disulfide bonds. Both the structure, determined by circular dichroism, and the biological activity, determined by toxicity assay, were remarkably resistant to heating.<\/jats:p><jats:p>In the rat phrenic nerve\u2010diaphragm preparation, the toxin caused a failure of neuromuscular transmission, after which subthreshold endplate potentials could be detected. The frequency of spontaneous miniature potentials was altered only to a very slight extent. The toxin appeared to have no postsynaptic activity. Similarities between the properties of this neurotoxin and that described by Lee and Chang (1966) suggest that it is identical to \u03b2\u2010bungarotoxin.<\/jats:p>","DOI":"10.1002\/neu.480050205","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T10:35:35Z","timestamp":1104575735000},"page":"135-150","source":"Crossref","is-referenced-by-count":83,"title":["Biochemical and physiological properties of a purified snake venom neurotoxin which acts presynaptically"],"prefix":"10.1002","volume":"5","author":[{"given":"Regis B.","family":"Kelly","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Frank R.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/234207a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3233(08)60022-4"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"4473","DOI":"10.1016\/S0021-9258(18)94342-5","article-title":"Isolation and characterization of Clostridium botulinum type B toxin","volume":"244","author":"Beers W. 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