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A considerable amount of free <jats:sup>3<\/jats:sup>H\u2010leucine is transported at constant velocity along the axon in the form of a distinct peak. The maximum transport velocity for free <jats:sup>3<\/jats:sup>H\u2010leucine is the same as for rapidly transported <jats:sup>3<\/jats:sup>H\u2010protein (130 and 135 mm\/day, respectively, at 19\u00b0C). Microtubule inhibitors block or significantly reduce the amount of free <jats:sup>3<\/jats:sup>H\u2010leucine transported, but do not influence the transport velocity. Disruption of the oxygen supply abolishes free <jats:sup>3<\/jats:sup>H\u2010leucine transport, so that this phenomenon cannot be explained by diffusion. The amount of free leucine in the rapidly moving peak decreases with time and distance along the axon and is not detectable after 5 h or more. The transported <jats:sup>3<\/jats:sup>H\u2010leucine is not derived from the circulation or from proteolysis of rapidly transported proteins. This study may help to resolve the controversy over the axoplasmic transport of free amino acids since it shows that free <jats:sup>3<\/jats:sup>H\u2010leucine is transported rapidly but does not travel by rapid axoplasmic transport to the end of axons longer than about 30 mm.<\/jats:p>","DOI":"10.1002\/neu.480140205","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T04:07:06Z","timestamp":1104552426000},"page":"133-144","source":"Crossref","is-referenced-by-count":13,"title":["Rapid axoplasmic transport of free leucine"],"prefix":"10.1002","volume":"14","author":[{"given":"Georg","family":"Schmid","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lothar","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dieter G.","family":"Weiss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(69)90032-X"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1974.tb11586.x"},{"key":"e_1_2_1_4_1","first-page":"425","article-title":"Axonal Transport\u2014I. 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