{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:05:13Z","timestamp":1760954713896},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,11]],"date-time":"2004-10-11T00:00:00Z","timestamp":1097452800000},"content-version":"vor","delay-in-days":5032,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Neurobiol."],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ependymin, a prominent protein of the brain's extracellular fluid (ECF) was\u2010previously implicated in the consolidation of memory and in the activity\u2010driven sharpening of the retinotectal projection. Because both these phenomena probably involve the growth and elaboration of appropriate synapses, we have tested whether ependymin can serve as a substrate for the growth of axons from goldfish retinal ganglion cells in a culture assay. Ependymin (Ep), laminin (LAM), polylysine (PL), and Concanavalin A (Con A) were plated on glass coverslips either uniformly or in striped patterns. Ep alone, either soluble or partly polymerized (by dropping calcium concentration and pH), was a good substrate for axonal outgrowth, as good or better than PL and Con A, but not as good as LAM. Neurites grew faster on LAM (71 \u03bcm\/h) than on Ep (32 \u03bcm\/h) or on PL (22 \u03bcm\/h). Fasciculation was low on LAM, intermediate on Ep, and highest on PL. In exclusive side\u2010by\u2010side stripe assays, axons preferred LAM over Ep, but gave weak or no preference for Ep over Con A or PL. With stripes of LAM + Ep alongside pure LAM, the axons preferred the mixture of LAM + Ep. When antibodies to Ep were plated in stripes over continuous Ep substrate, the axons avoided the antibody\u2010blocked stripes and grew on the Ep stripes. Antibodies to Ep did not, however, block growth on laminin substrates, nor did antibodies to LAM block growth on Ep. Dot blots and western blots showed very little cross recognition between the antibodies. Ependymin is a good substrate for neurite outgrowth, which is normally present in ECF, and adhesion to Ep is independent of LAM and possibly additive to it.<\/jats:p>","DOI":"10.1002\/neu.480220105","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T03:09:29Z","timestamp":1104548969000},"page":"40-54","source":"Crossref","is-referenced-by-count":24,"title":["Ependymin as a substrate for outgrowth of axons from cultured explants of goldfish retina"],"prefix":"10.1002","volume":"22","author":[{"given":"John T.","family":"Schmidt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rupert","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weichun","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoying","family":"Jian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia A. O.","family":"Stuermer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.7.2373"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(89)90518-1"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(77)90800-9"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7233208"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90314-9"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.141.3575.57"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/322419a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(88)90139-7"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.05-11-03030.1985"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1989.tb10932.x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1139\/y82-243"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/311153a0"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/321519a0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90796-X"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(82)90064-1"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(88)90429-0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3775383"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1986.tb27169.x"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)91431-X"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(83)90350-0"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.45.030183.003053"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.480210608"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90886-4"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902690408"},{"key":"e_1_2_1_27_1","first-page":"725","article-title":"Long\u2010term memory and morphological distribution of involved proteins in goldfish brain","volume":"77","author":"Schmidt R.","year":"1983","journal-title":"Verh. Anat. Ges."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-034186-6.50041-4"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1987.tb05750.x"},{"key":"e_1_2_1_30_1","first-page":"327","volume-title":"Fundamentals of Memory Formation: Neural Plasticity and Brain Function. Progr. Zool","author":"Schmidt R.","year":"1989"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)90161-7"},{"key":"e_1_2_1_32_1","volume-title":"Structure and Development of the Forebrain in Lower Vertebrates","author":"Schmidt R."},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1981.tb00574.x"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1983.tb08030.x"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-73160-0_19"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(76)90779-4"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00711092"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00973283"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(86)80581-2"},{"key":"e_1_2_1_40_1","first-page":"782","article-title":"Role of brain extracellular proteins in the mechanism of long\u2010term potentiation in rat brain hippocampus","volume":"11","author":"Halvorsen S. W.","year":"1985","journal-title":"Neurosci. Abstr."},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)90377-6"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)91460-X"},{"key":"e_1_2_1_43_1","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1111\/j.1471-4159.1986.tb00674.x","article-title":"Extracellular fluid proteins of goldfish brain: Evidence for the presence of proteases and esterases","volume":"47","author":"Halvorsen S. W.","year":"1986","journal-title":"J. Neurochem."},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(78)90731-X"},{"key":"e_1_2_1_45_1","first-page":"87","volume-title":"Dynamics and Plasticity of Neuronal Systems","author":"Stuermer C. A. O.","year":"1989"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902230106"},{"key":"e_1_2_1_47_1","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications","volume":"76","author":"Towbin H.","year":"1979","journal-title":"PNASUSA"},{"key":"e_1_2_1_48_1","first-page":"167","article-title":"In vitro assay to test the differential substrate affinity of growing axons","volume":"26","author":"Vielmetter J.","year":"1986","journal-title":"Neurosci. Lett."},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90071-8"},{"key":"e_1_2_1_50_1","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1242\/dev.101.4.685","article-title":"Recognition of position specific properties of tectal cell membranes by retinal axons in vitro","volume":"101","author":"Walter J.","year":"1987","journal-title":"Development"}],"container-title":["Journal of Neurobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fneu.480220105","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/neu.480220105","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T23:29:48Z","timestamp":1698017388000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/neu.480220105"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,1]]},"references-count":49,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,1]]}},"alternative-id":["10.1002\/neu.480220105"],"URL":"https:\/\/doi.org\/10.1002\/neu.480220105","archive":["Portico"],"relation":{},"ISSN":["0022-3034","1097-4695"],"issn-type":[{"value":"0022-3034","type":"print"},{"value":"1097-4695","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,1]]}}}